Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.105 extracted from

  • Duran, J.; Navarro-Sabate, A.; Pujol, A.; Perales, J.C.; Manzano, A.; Obach, M.; Gomez, M.; Bartrons, R.
    Overexpression of ubiquitous 6-phosphofructo-2-kinase in the liver of transgenic mice results in weight gain (2008), Biochem. Biophys. Res. Commun., 365, 291-297.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, overexpression under the control of the phosphoenolpyruvate carboxykinase promoter in Mus musculus, microinjection of fertilized eggs, C57BL56/SJL receptor females of Mus musculus implanted with microinjected oocytes Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16875 Pfkfb3 gene; overexpression of the Pfkfb3 gene in Mus musculus, generation of uPFK-2 transgenic line
-

Source Tissue

Source Tissue Comment Organism Textmining
liver transgenic line of Mus musculus Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
diet-induced obesity in transgenic mice, liver and plasma metabolites in transgenic and control mice, human Pfkfb3-specific gene probe used to quantify transgene overexpression by RT-PCR, measurement of fructose 2,6-bisphosphate levels in the liver, immunostaining and gene expression analysis in livers of transgenic and control mice, changes in hepatic gene expression profiles for key gluconeogenic and lipogenic enzymes, accumulation of lipids in periportal cells, gain in weight Homo sapiens