Literature summary for 2.7.1.105 extracted from

Mukhtar, M.H.; Payne, V.A.; Arden, C.; Harbottle, A.; Khan, S.; Lange, A.J.; Agius, L.
Inhibition of glucokinase translocation by AMP-activated protein kinase is associated with phosphorylation of both GKRP and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (2008), Am. J. Physiol. Regul. Integr. Comp. Physiol., 294, R766-R774.

Search for more literature for 2.7.1.105

Application

Application	Comment	Organism
pharmacology	effects of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR) on hepatic glucose metabolism	Rattus norvegicus

Cloned(Commentary)

Cloned (Comment)	Organism
wild-type and mutant protein, expression via adenovirus transfection system in hepatocytes	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside	AICAR, associated with phosphorylation of PFK-2 on Ser-32, phosphorylation increased of both wild-type and overexpressed PFK-2 protein in hepatocytes	Rattus norvegicus
glucagon	phosphorylation of PFK2 on Ser-32 in liver	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + beta-D-fructose 6-phosphate	Rattus norvegicus	-	ADP + beta-D-fructose 2,6-bisphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	Q9JJH5	male, Wistar background, primary culture of hepatocytes	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	Ser-32 is the phophorylation site	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
hepatocyte	primary culture, treated and untreated with adenoviral vector to overexpress wild-type PFK-2 and mutant PKF2-M lacking Ser-32 phosphorylation site	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	inhibition of glucose-induced translocation, 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR) associated with phosphorylation of PFK2 on Ser-32, increase of phosphorylation less than that caused by glucagon, expression of PFK-2 mutant that lacks Ser-32 (PFK2-M) reverses inhibitory effects on glucokinase translocation	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + beta-D-fructose 6-phosphate	-	Rattus norvegicus	ADP + beta-D-fructose 2,6-bisphosphate	-	?
ATP + beta-D-fructose 6-phosphate	phosphorylation of PFK-2 on Ser-32, inhibition of hepatic glucose metabolism	Rattus norvegicus	ADP + beta-D-fructose 2,6-bisphosphate	-	?

Synonyms

Synonyms	Comment	Organism
Pfk-2	-	Rattus norvegicus

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Release 2023.1 (January 2023)