Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in human HEK-293 cells via adenovirus transfection system, overexpression of the mutant enzyme highly increase the endogenous level of glycolysis and fructose 2,6-bisphosphate in presence of glucagon | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
S32A/H258A | site-directed mutagenesis, increased activity and incresaed expression level in recombinant HEK-293 cells compared to the wild-type enzyme | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glucagon | the native and the recombinant wild-type and mutant enzymes are phosphorylated after incubation with glucagon inactivating the enzyme | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | Rattus norvegicus | - |
ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
additional information | Rattus norvegicus | role of the enzyme in catalysis and regulation of glycolysis/gluconeogenesis and related enzymes, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Wistar rats | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the native and the recombinant wild-type and mutant enzymes are phosphorylated after incubation with glucagon inactivating the enzyme | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | bifunctional enzyme comprises both 6-phosphofructo-2-kinase and fructose-2,6-bisphosphatase, EC 3.1.3.46, activities | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Rattus norvegicus | - |
hepatocyte | primary culture | Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Rattus norvegicus | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
ATP + beta-D-fructose 6-phosphate | bifunctional enzyme catalyzes the forward and reverse reaction using different catalytic sites | Rattus norvegicus | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
additional information | role of the enzyme in catalysis and regulation of glycolysis/gluconeogenesis and related enzymes, overview | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PFK2 | - |
Rattus norvegicus |
phosphofructokinase-2/fructose bisphosphatase-2 | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |