Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyruvate | - |
Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the deletion of N-terminal 318 amino acids abolishes the Michaelis-Menten kinetics of enzyme, Km of beta-D-fructose 6-phosphate is increased, whereas Km of ATP is not significantly altered. When the first 66 amino acids are deleted, the activity ration between 6-phosphofructo-2-kinase and beta-D-fructose 2,6-bisphosphatase is halved. The deletion of 125, 179, 249 and 318 amino acids results in progressive further decreases in the activity ratio and the activity ratio is reduced 4fold when N-terminus is deleted completely. The full-length enzyme is eluted as a tetramer, whereas the truncated enzymes are eluted as monomers | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Arabidopsis thaliana | |
AMP | not | Arabidopsis thaliana | |
ATP | not | Arabidopsis thaliana | |
dihydroxyacetone phosphate | - |
Arabidopsis thaliana | |
diphosphate | not phosphate | Arabidopsis thaliana | |
glycerate 2-phosphate | - |
Arabidopsis thaliana | |
phosphoenolpyruvate | - |
Arabidopsis thaliana | |
sn-glycerol 3-phosphate | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | comparison of Km of wild-type and mutant enzyme | Arabidopsis thaliana | |
0.19 | - |
ATP | pH 6.0, 25°C | Arabidopsis thaliana | |
0.5 | - |
beta-D-fructose 6-phosphate | pH 6.0, 25°C | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
4 * 83000 | Arabidopsis thaliana |
370000 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | Arabidopsis thaliana | - |
ADP + beta-D-fructose 2,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9MB58 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Arabidopsis thaliana | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
ATP + beta-D-fructose 6-phosphate | - |
Arabidopsis thaliana | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 83000 | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
10 min, enzyme is stable, both in the absence and in the presence of phosphate | Arabidopsis thaliana |
25 | - |
about 76% of enzyme activity remains after 10 min, both in the absence and in the presence of phosphate | Arabidopsis thaliana |
42 | - |
10 min, activation of enzyme and deletion mutant decreases rapidly, but the presence of phosphate protectes the enzyme activities from inactivation and 75% of enzyme activity remains after 15 min | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
phosphoenolpyruvate | pH 6.0, 25°C | Arabidopsis thaliana | |
0.35 | - |
sn-glycerol 3-phosphate | pH 6.0, 25°C | Arabidopsis thaliana | |
0.45 | - |
glycerate 2-phosphate | pH 6.0, 25°C | Arabidopsis thaliana | |
1.35 | - |
dihydroxyacetone phosphate | pH 6.0, 25°C | Arabidopsis thaliana |