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Literature summary for 2.7.1.105 extracted from

  • Villadsen, D.; Nielsen, T.H.
    N-terminal truncation affects the kinetics and structure of fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase from Arabidopsis thaliana (2001), Biochem. J., 359, 591-597.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
pyruvate
-
Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information the deletion of N-terminal 318 amino acids abolishes the Michaelis-Menten kinetics of enzyme, Km of beta-D-fructose 6-phosphate is increased, whereas Km of ATP is not significantly altered. When the first 66 amino acids are deleted, the activity ration between 6-phosphofructo-2-kinase and beta-D-fructose 2,6-bisphosphatase is halved. The deletion of 125, 179, 249 and 318 amino acids results in progressive further decreases in the activity ratio and the activity ratio is reduced 4fold when N-terminus is deleted completely. The full-length enzyme is eluted as a tetramer, whereas the truncated enzymes are eluted as monomers Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Arabidopsis thaliana
AMP not Arabidopsis thaliana
ATP not Arabidopsis thaliana
dihydroxyacetone phosphate
-
Arabidopsis thaliana
diphosphate not phosphate Arabidopsis thaliana
glycerate 2-phosphate
-
Arabidopsis thaliana
phosphoenolpyruvate
-
Arabidopsis thaliana
sn-glycerol 3-phosphate
-
Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information comparison of Km of wild-type and mutant enzyme Arabidopsis thaliana
0.19
-
ATP pH 6.0, 25°C Arabidopsis thaliana
0.5
-
beta-D-fructose 6-phosphate pH 6.0, 25°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
phosphate
-
Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
4 * 83000 Arabidopsis thaliana
370000
-
gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + beta-D-fructose 6-phosphate Arabidopsis thaliana
-
ADP + beta-D-fructose 2,6-bisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9MB58
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-fructose 6-phosphate
-
Arabidopsis thaliana ADP + beta-D-fructose 2,6-bisphosphate
-
?
ATP + beta-D-fructose 6-phosphate
-
Arabidopsis thaliana ADP + beta-D-fructose 2,6-bisphosphate
-
r
additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 83000 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
10 min, enzyme is stable, both in the absence and in the presence of phosphate Arabidopsis thaliana
25
-
about 76% of enzyme activity remains after 10 min, both in the absence and in the presence of phosphate Arabidopsis thaliana
42
-
10 min, activation of enzyme and deletion mutant decreases rapidly, but the presence of phosphate protectes the enzyme activities from inactivation and 75% of enzyme activity remains after 15 min Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.13
-
phosphoenolpyruvate pH 6.0, 25°C Arabidopsis thaliana
0.35
-
sn-glycerol 3-phosphate pH 6.0, 25°C Arabidopsis thaliana
0.45
-
glycerate 2-phosphate pH 6.0, 25°C Arabidopsis thaliana
1.35
-
dihydroxyacetone phosphate pH 6.0, 25°C Arabidopsis thaliana