Cloned (Comment) | Organism |
---|---|
mutant expressed in Escherichia coli BL21(DL3) | Homo sapiens |
mutant expressed in Escherichia coli BL21(DL3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
P2R | kinetic properties of human liver mutant and rat wild-type enzyme are very similar | Homo sapiens |
P2R | kinetic properties of human liver mutant and rat wild-type enzyme are very similar | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | 0.021 | beta-D-fructose 6-phosphate | pH 7.8, in presence of phosphate | Homo sapiens | |
0.019 | 0.021 | beta-D-fructose 6-phosphate | pH 7.8, in presence of phosphate | Rattus norvegicus | |
0.12 | - |
beta-D-fructose 6-phosphate | pH 7.8 | Rattus norvegicus | |
0.12 | 0.36 | ATP | pH 7.8 | Rattus norvegicus | |
0.4 | - |
ATP | - |
Rattus norvegicus | |
0.4 | - |
ATP | pH 7.8 | Homo sapiens | |
1 | 1.1 | beta-D-fructose 6-phosphate | pH 7.8, absence of phosphate | Homo sapiens | |
1 | 1.1 | beta-D-fructose 6-phosphate | pH 7.8, absence of phosphate | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Homo sapiens | |
phosphate | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
primary structures of human, rat and bovine liver enzyme | Homo sapiens |
additional information | - |
primary structures of human, rat and bovine liver enzyme | Rattus norvegicus |
55000 | - |
2 * 55000, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human enzyme is 95% identical with rat or bovine enzyme | - |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 | Homo sapiens | |
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.046 | - |
- |
Homo sapiens |
0.046 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Homo sapiens | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
ATP + beta-D-fructose 6-phosphate | - |
Rattus norvegicus | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Homo sapiens | ? | - |
? | |
additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Homo sapiens |
dimer | 2 * 55000, SDS-PAGE | Rattus norvegicus |