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Literature summary for 2.7.1.1 extracted from
- Molecular cloning and characterization of Brugia malayi hexokinase (2008), Parasitol. Int., 57, 354-361.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glucose-6-phosphate | 10 mM, recombinant enzyme is 3fold activated | Brugia malayi |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Brugia malayi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 20 mM, 96% inhibition | Brugia malayi | |
| ADP | 0.0001 mM, 50% inhibition. Noncompetitive inhibition with the substrate glucose | Brugia malayi | |
| CaCl2 | 10 mM, 98% inhibition | Brugia malayi | |
| D-glucosamine | - |
Brugia malayi | |
| D-glucose 6-phosphate | - |
Brugia malayi | |
| diphosphate | mixed type of inhibition when ATP is used as substrate | Brugia malayi | |
| EDTA | 40 mM, 91% inhibition | Brugia malayi | |
| KCl | 10 mM, 92% inhibition | Brugia malayi | |
| mannoheptulose | - |
Brugia malayi | |
| Mg2+ | 100 mM, 72% inhibition | Brugia malayi | |
| N-acetyl glucosamine | - |
Brugia malayi | |
| NaCl | 10 mM, 96% inhibition | Brugia malayi | |
| p-chloromercuribenzoate | 0.05 mM, 72% inhibition | Brugia malayi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
D-glucose | - |
Brugia malayi | |
| 1.09 | - |
ATP | - |
Brugia malayi | |
| 75 | - |
D-fructose | - |
Brugia malayi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
4 * 72000, SDS-PAGE | Brugia malayi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brugia malayi | Q56VN6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Brugia malayi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | - |
Brugia malayi | ADP + D-fructose 6-phosphate | - |
? | |
| ATP + D-galactose | - |
Brugia malayi | ? | - |
? | |
| ATP + D-glucose | - |
Brugia malayi | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-maltose | - |
Brugia malayi | ? | - |
? | |
| ATP + D-mannose | - |
Brugia malayi | ADP + D-mannose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 72000, SDS-PAGE | Brugia malayi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BmHk | - |
Brugia malayi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Brugia malayi |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
diphosphate | - |
Brugia malayi | |
| 0.55 | - |
ADP | substrate glucose | Brugia malayi | |
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