Literature summary for 2.7.1.1 extracted from

Claeyssen, E.; Wally, O.; Matton, D.P.; Morse, D.; Rivoal, J.
Cloning, expression, purification, and properties of a putative plasma membrane hexokinase from Solanum chacoense (2006), Protein Expr. Purif., 47, 329-339.

Search for more literature for 2.7.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed as a hexahistidine-tagged protein in Escherichia coli	Solanum chacoense

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	0.5 mM, 43% inhibition	Solanum chacoense
D-glucosamine	20 mM, 67% inhibition	Solanum chacoense
D-mannose	10 mM, 88% loss of activity	Solanum chacoense

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.023	-	D-glucose	30°C, pH 8.0	Solanum chacoense
0.03	-	D-mannose	30°C, pH 8.0	Solanum chacoense
0.061	-	ATP	30°C, pH 8.0	Solanum chacoense
0.231	-	GTP	30°C, pH 8.0	Solanum chacoense
0.231	-	TTP	30°C, pH 8.0	Solanum chacoense
2	-	CTP	30°C, pH 8.0	Solanum chacoense
2.2	-	UTP	30°C, pH 8.0	Solanum chacoense
5.2	-	D-fructose	30°C, pH 8.0	Solanum chacoense

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	-	Solanum chacoense	5886	-

Organism

Organism	UniProt	Comment	Textmining
Solanum chacoense	Q3S2I3	-	-

Purification (Commentary)

Purification (Comment)	Organism
full length recombinant protein	Solanum chacoense

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.3	-	-	Solanum chacoense

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-fructose	-	Solanum chacoense	ADP + D-fructose 6-phosphate	-	?
ATP + D-glucose	-	Solanum chacoense	ADP + D-glucose 6-phosphate	-	?
ATP + D-mannose	-	Solanum chacoense	ADP + D-mannose 6-phosphate	-	?
CTP + D-glucose	-	Solanum chacoense	CDP + D-glucose 6-phosphate	-	?
GTP + D-glucose	-	Solanum chacoense	GDP + D-glucose 6-phosphate	-	?
TTP + D-glucose	-	Solanum chacoense	TDP + D-glucose 6-phosphate	-	?
UTP + D-glucose	-	Solanum chacoense	UDP + D-glucose 6-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
ScHK2	-	Solanum chacoense

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.85	-	GTP	30°C, pH 8.0	Solanum chacoense
1.42	-	TTP	30°C, pH 8.0	Solanum chacoense
1.61	-	CTP	30°C, pH 8.0	Solanum chacoense
3.02	-	UTP	30°C, pH 8.0	Solanum chacoense
3.3	-	D-glucose	30°C, pH 8.0	Solanum chacoense
3.5	-	ATP	30°C, pH 8.0	Solanum chacoense
3.69	-	D-fructose	30°C, pH 8.0	Solanum chacoense
3.78	-	D-mannose	30°C, pH 8.0	Solanum chacoense
5.01	-	D-glucose	30°C, pH 8.0	Solanum chacoense

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.7	-	-	Solanum chacoense

pH Range

pH Minimum	pH Maximum	Comment	Organism
7.7	8.7	ScHK2 activity decreases sharply (3fold) within a narrow range from pH 8.7 down to pH 7.7	Solanum chacoense

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Release 2023.1 (January 2023)