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Literature summary for 2.7.1.1 extracted from

  • Pino, M.F.; Kim, K.A.; Shelton, K.D.; Lindner, J.; Odili, S.; Li, C.; Collins, H.W.; Shiota, M.; Matschinsky, F.M.; Magnuson, M.A.
    Glucokinase thermolability and hepatic regulatory protein binding are essential factors for predicting the blood glucose phenotype of missense mutations (2007), J. Biol. Chem., 282, 13906-13916.
    View publication on PubMed
Search for more literature for 2.7.1.1

Protein Variants

Protein Variants Comment Organism
A456V mutant enzyme is as stable as the wild-type enzyme. Ability of glucokinase regulatory protein to inhibit mutant enzyme A456V is less than that of the wild type enzyme. Mutant shows 37.9% of the wild-type activity. KM-value for ATP is 1.3fold lower than wild-type value. Glucokinase A456V/A456V mice are viable and have similar plasma triglycerides, free fatty acid levels, and similar or slightly lower body weight when compared with control mice Mus musculus
K414E mutant enzyme is thermolabile. Mutant shows 0.32% of the wild-type activity. KM-value for ATP is 4.4fold higher than wild-type value. Glucokinase K414E/K414E mice die within a few days after birth of severe hyperglycemia Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
 ATP 37°C, pH 7.4, mutant enzyme A456V Mus musculus
0.39
-
 ATP 37°C, pH 7.4, wild-type enzyme Mus musculus
1.73
-
 ATP 37°C, pH 7.4, mutant enzyme K414E Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mus musculus 5829
-

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
-
 Mus musculus ADP + D-glucose 6-phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 3.7 ATP 37°C, pH 7.4, mutant enzyme A456V Mus musculus
2 3.7 D-glucose 37°C, pH 7.4, mutant enzyme A456V Mus musculus
11
-
 ATP 37°C, pH 7.4, mutant enzyme K414E Mus musculus
11
-
 D-glucose 37°C, pH 7.4, mutant enzyme K414E Mus musculus
53.2
-
 ATP 37°C, pH 7.4, wild-type enzyme Mus musculus
53.2
-
 D-glucose 37°C, pH 7.4, wild-type enzyme Mus musculus
84.9
-
 ATP 37°C, pH 7.4, mutant enzyme A456V Mus musculus
84.9
-
 D-glucose 37°C, pH 7.4, mutant enzyme A456V Mus musculus