Cloned (Comment) | Organism |
---|---|
type I enzyme, DNA and amino acid sequence determination and analysis, functional expression of soluble full length enzyme and of the soluble C-terminal domain in Escherichia coli strain BL21(DE3) | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-Glucose 1,6-bisphosphate | competitive versus MgATP2-, low concentration of phosphate counteract | Bos taurus | |
D-glucose 6-phosphate | competitive versus MgATP2- | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
D-glucose | pH 7.2, 37°C, recombinant catalytic C-terminal domain | Bos taurus | |
0.072 | - |
D-glucose | pH 7.2, 37°C, recombinant full length enzyme | Bos taurus | |
0.67 | - |
ATP | pH 7.2, 37°C, recombinant full length enzyme | Bos taurus | |
0.67 | - |
ATP | ATP in form of MgATP2- | Bos taurus | |
0.98 | - |
ATP | pH 7.2, 37°C, recombinant catalytic C-terminal domain | Bos taurus | |
0.98 | - |
ATP | ATP in form of MgATP2- | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | MgATP2- | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 111000, recombinant full length enzyme, SDS-PAGE, x * 56000, recombinant catalytic C-terminal domain, SDS-PAGE | Bos taurus |
111000 | - |
x * 111000, recombinant full length enzyme, SDS-PAGE, x * 56000, recombinant catalytic C-terminal domain, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | Bos taurus | involved in glucose catabolism | ADP + D-glucose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | Q5W5U3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant full length enzyme and C-terminal domain from Escherichia coli strain BL21(DE3) by ion exchange, hydrophobic interaction, anddye ligand affinity chromatography, and gel filtration | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.44 | - |
cell extract of recombinant Escherichia coli expressing the full length enzyme | Bos taurus |
3.5 | - |
cell extract of recombinant Escherichia coli expressing the catalytic C-terminal domain of the enzyme | Bos taurus |
94 | - |
purified recombinant full length enzyme | Bos taurus |
141 | - |
purified recombinant catalytic C-terminal domain of the enzyme | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-2-fluoro-D-glucose | - |
Bos taurus | ADP + 2-deoxy-2-fluoro-D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | - |
Bos taurus | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | involved in glucose catabolism | Bos taurus | ADP + D-glucose 6-phosphate | - |
? | |
additional information | the active site is located in the C-terminal domain | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 111000, recombinant full length enzyme, SDS-PAGE, x * 56000, recombinant catalytic C-terminal domain, SDS-PAGE | Bos taurus |
More | the active site is located in the C-terminal domain | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
hexokinase type I | - |
Bos taurus |
HK1 | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | MgATP2- | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
D-Glucose 1,6-bisphosphate | pH 7.2, 37°C, recombinant catalytic C-terminal domain, versus MgATP2- | Bos taurus | |
0.036 | - |
D-Glucose 1,6-bisphosphate | pH 7.2, 37°C, recombinant full length enzyme, versus MgATP2- | Bos taurus | |
0.045 | - |
D-glucose 6-phosphate | pH 7.2, 37°C, recombinant full length enzyme | Bos taurus | |
0.066 | - |
D-glucose 6-phosphate | pH 7.2, 37°C, recombinant catalytic C-terminal domain | Bos taurus |