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Literature summary for 2.7.1.1 extracted from

  • Sindelar, L.; Sindelarova, M.
    Hexokinases of tobacco leaves: Changes in the cytosolic and non-cytosolic isozyme complexes induced by tobacco mosaic virus infection (2003), Biol. Plant., 47, 413-419.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
additional information increased activity of the cytosolic and non-cytosolic isozymes induced by tobacco mosaic virus TMV Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol 2 soluble isozymes, increased activity of the cytosolic and non-cytosolic isozymes induced by tobacco mosaic virus infection Nicotiana tabacum 5829
-
particle-bound 1 particulate isozyme, increased activity of the cytosolic and non-cytosolic isozymes induced by tobacco mosaic virus infection Nicotiana tabacum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose Nicotiana tabacum
-
ADP + D-fructose 6-phosphate
-
?
ATP + D-glucose Nicotiana tabacum
-
ADP + D-glucose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
cv. Samsun, 3 isozymes: 1 membrane-bound, 2 soluble
-

Purification (Commentary)

Purification (Comment) Organism
partially, subcellular fractionation by anion exchange chromatography of isozymes from healthy and TMV-infected leaves Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
leaf changes in the cytosolic and non-cytosolic isozyme complexes induced by tobacco mosaic virus infection Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.114
-
healthy leaves, soluble glucokinase activity Nicotiana tabacum
0.199
-
TMV-infected leaves, soluble glucokinase activity Nicotiana tabacum
0.574
-
healthy leaves, particulate glucokinase activity Nicotiana tabacum
0.609
-
healthy leaves, soluble fructokinase activity Nicotiana tabacum
0.688
-
healthy leaves, particulate fructokinase activity Nicotiana tabacum
0.688
-
healthy leaves, total glucokinase activity Nicotiana tabacum
0.693
-
TMV-infected leaves, particulate glucokinase activity Nicotiana tabacum
0.892
-
TMV-infected leaves, total glucokinase activity Nicotiana tabacum
1.297
-
healthy leaves, total fructokinase activity Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose
-
Nicotiana tabacum ADP + D-fructose 6-phosphate
-
?
ATP + D-glucose
-
Nicotiana tabacum ADP + D-glucose 6-phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
ATP
-
Nicotiana tabacum