Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.1 extracted from

  • Caceres, A.J.; Portillo, R.; Acosta, H.; Rosales, D.; Quinones, W.; Avilan, L.; Salazar, L.; Dubourdieu, M.; Michels, P.A.M.; Concepcion, J.L.
    Molecular and biochemical characterization of hexokinase from Trypanosoma cruzi (2003), Mol. Biochem. Parasitol., 126, 251-262.
    View publication on PubMed
Search for more literature for 2.7.1.1

Application

Application Comment Organism
pharmacology enzyme may constitute an interesting drug target Trypanosoma cruzi

Cloned(Commentary)

Cloned (Comment) Organism
hexokinase gene is cloned, sequenced and expressed in Escherichia coli XL-1 Blue, gene encodes a polypeptide of 471 amino acids Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
ADP natural hexokinase from epimastigotes Trypanosoma cruzi
diphosphate natural hexokinase from epimastigotes, mixed-type inhibition Trypanosoma cruzi
additional information not inhibited by D-fructose; not inhibited by D-glucose 6-phosphate; not inhibited by trehalose 6-phosphate Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 D-glucose natural hexokinase from epimastigotes, at pH 7.5 Trypanosoma cruzi
0.06
-
 D-glucose recombinant hexokinase expressed in Escherichia coli XL-1 Blue, at pH 7.5 Trypanosoma cruzi
0.32 0.38 ATP natural and recombinant hexokinase, expressed in Escherichia coli XL-1 Blue, at pH 7.5 Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
glycosome in glycosome-enriched fraction Trypanosoma cruzi 20015
-
additional information sequence contains an N-terminal peroxisome-targeting signal, PTS-2, subcellular distribution Trypanosoma cruzi
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ MgCl2 is most effective, followed by MnCl2 with 16% of the activity found with MgCl2 Trypanosoma cruzi
Mg2+ strict requirement for divalent metal ions Trypanosoma cruzi
Mn2+ MgCl2 is most effective, followed by MnCl2 with 16% of the activity found with MgCl2 Trypanosoma cruzi
Mn2+ strict requirement for divalent metal ions Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 2 * 49000, at high ionic strength the enzyme exists as dimer, SDS-PAGE Trypanosoma cruzi
49000
-
 4 * 52000, predominant form, enzyme exists in several aggregation states, calculated from the amino acid sequence, 4 * 49000, SDS-PAGE Trypanosoma cruzi
52000
-
 4 * 52000, predominant form, enzyme exists in several aggregation states, calculated from the amino acid sequence, 4 * 49000, SDS-PAGE Trypanosoma cruzi
100000
-
 natural hexokinase, dimer, at high ionic strength, enzyme exists in several aggregation states Trypanosoma cruzi
180000
-
 recombinant hexokinase expressed in Escherichia coli XL-1 Blue, at low ionic strength, gel filtration Trypanosoma cruzi
200000
-
 gel filtration Trypanosoma cruzi
200000
-
 natural hexokinase, tetramer, at low ionic strength, enzyme exists in several aggregation states Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + ATP Trypanosoma cruzi glycolytic enzyme D-glucose 6-phosphate + ADP
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q8ST54 strains Y and EP, similar to hexokinase IV
-

Purification (Commentary)

Purification (Comment) Organism
48.6fold: natural hexokinase from epimastigotes, 66.3fold: recombinant hexokinase expressed in Escherichia coli XL-1 Blue Trypanosoma cruzi

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
 Trypanosoma cruzi
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.27
-
 recombinant hexokinase expressed in Escherichia coli XL-1 Blue, at 25°C, pH 7.5 Trypanosoma cruzi
19.44
-
 natural hexokinase from epimastigotes, at 25°C, pH 7.5 Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + ATP specific for glucose Trypanosoma cruzi ADP + D-glucose 6-phosphate
-
 ?
D-glucose + ATP glycolytic enzyme Trypanosoma cruzi D-glucose 6-phosphate + ADP
-
 ?
additional information not: diphosphate Trypanosoma cruzi ?
-
 ?
additional information not: mannose Trypanosoma cruzi ?
-
 ?
additional information not: fructose Trypanosoma cruzi ?
-
 ?
additional information not: D-galactose Trypanosoma cruzi ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 49000, at high ionic strength the enzyme exists as dimer, SDS-PAGE Trypanosoma cruzi
tetramer 4 * 52000, predominant form, enzyme exists in several aggregation states, calculated from the amino acid sequence, 4 * 49000, SDS-PAGE Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information natural hexokinase: 68160/min, recombinant hexokinase expressed in Escherichia coli XL-1 Blue: 18913/min, at pH 7.5 Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Trypanosoma cruzi
7.5 8.8
-
 Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Trypanosoma cruzi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.5
-
 diphosphate natural hexokinase from epimastigotes, at pH 7.5 Trypanosoma cruzi
0.7
-
 diphosphate recombinant hexokinase expressed in Escherichia coli, at pH 7.5 Trypanosoma cruzi
1.3
-
 ADP natural hexokinase from epimastigotes, at pH 7.5 Trypanosoma cruzi

pI Value

Organism Comment pI Value Maximum pI Value
Trypanosoma cruzi calculated basic isoelectric point
-
 9.67