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Literature summary for 2.7.1.1 extracted from

  • Bianchi, M.; Serafini, G.; Bartolucci, E.; Giammarini, C.; Magnani, M.
    Enzymatic properties of overexpressed human hexokinase fragments (1998), Mol. Cell. Biochem., 189, 185-193.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning and overexpression of a full-length HK I, a truncate form lacking the first 11 amino acids named HK-11aa, and of the 50 kDa C-terminal half containing the catalytic domain in Escherichia coli BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
D-glucose 1,6-diphosphate strong inhibition of a recombinant full-length HK I, a truncated form lacking the first 11 amino acids named HK-11aa, and of the 50 kDa C-terminal half containing the catalytic domain, strongly pH-dependent, inhibition is reversed by phosphate, except that of the C-terminal half Homo sapiens
D-glucose 6-phosphate competitive to MgATP2-; strong inhibition of a recombinant full-length HK I, a truncated form lacking the first 11 amino acids named HK-11aa, and of the 50 kDa C-terminal half containing the catalytic domain, at low concentrations, 0-3 mM, inhibition is reversed by phosphate, except that of the C-terminal half Homo sapiens
phosphate at high concentrations, 10-50 mM, inhibition of recombinant full-length HK I, a truncated form lacking the first 11 amino acids named HK-11aa, and of the 50 kDa C-terminal half containing the catalytic domain, competitive versus MgATP2- Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06 0.065 D-glucose recombinant HK I, a truncate HK I form lacking the first 11 amino acids named HK-11aa, and the 50 kDa C-terminal half of HK I, at 37°C, pH 7.2 Homo sapiens
1.1 1.4 ATP MgATP2-, recombinant HK I, a truncate HK I form lacking the first 11 amino acids named HK-11aa, and the 50 kDa C-terminal half of HK I, at 37°C, pH 7.2 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
recombinant hexokinase I
-

Purification (Commentary)

Purification (Comment) Organism
recombinant full-length HK I, truncate form lacking the first 11 amino acids named HK-11aa, 50 kDa C-terminal half containing the catalytic domain Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
the first 11 N-terminal amino acids influence the specific activity of HK Homo sapiens
2
-
unpurified recombinant HK I and 50 kDa C-terminal half of HK I, at 22°C Homo sapiens
3.8
-
unpurified recombinant HK-11aa, a truncated HK I form lacking the first 11 amino acids, at 22°C Homo sapiens
144
-
purified recombinant HK I, at 22°C Homo sapiens
180
-
purified recombinant HK-11aa, a truncated HK I form lacking the first 11 amino acids, at 22°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + ATP MgATP2- Homo sapiens ADP + D-glucose 6-phosphate
-
?
additional information the 50 kDa C-terminal half of HK I contains the catalytic domain, the N-terminal half is catalytically inactive Homo sapiens ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.029 0.037 D-glucose 6-phosphate recombinant HK I, a truncate HK I form lacking the first 11 amino acids named HK-11aa, and the 50 kDa C-terminal half of HK I, at 37°C, pH 7.2 Homo sapiens
0.05 0.059 D-glucose 1,6-diphosphate recombinant HK I, a truncate HK I form lacking the first 11 amino acids named HK-11aa, and the 50 kDa C-terminal half of HK I, at 37°C, pH 7.2 Homo sapiens
33
-
phosphate recombinant HK I, a truncate HK I form lacking the first 11 amino acids named HK-11aa, and the 50 kDa C-terminal half of HK I, at 37°C, pH 7.2 Homo sapiens