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Literature summary for 2.7.1.1 extracted from
- The hexokinase of the hyperthermophile Thermoproteus tenax. ATP-dependent hexokinases and ADP-dependent glucokinases, two alternatives for glucose phosphorylation in Archaea (2003), J. Biol. Chem., 278, 18744-18753.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| hxk gene encoding hexokinase is cloned, sequenced and expressed in Escherichia coli, member of the so-called ROK family, genomic organization of the orfX-hxk operon, hxk gene encodes a 301 amino acids protein | Thermoproteus tenax |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Thermoproteus tenax |  |
| AMP | - |
Thermoproteus tenax | |
| D-glucose 6-phosphate | - |
Thermoproteus tenax | |
| diphosphate | 1 mM, 70% inhibition | Thermoproteus tenax | |
| EDTA | 2.5 mM, complete inhibition | Thermoproteus tenax | |
| phosphate | - |
Thermoproteus tenax | |
| UDP | - |
Thermoproteus tenax | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.043 | - |
D-mannose | at 50°C, pH 7.5 | Thermoproteus tenax | |
| 0.058 | - |
D-glucose | at 50°C, pH 7.5 | Thermoproteus tenax | |
| 0.187 | - |
2-deoxy-D-glucose | at 50°C, pH 7.5 | Thermoproteus tenax | |
| 0.29 | - |
ATP | at 50°C, pH 7.5 | Thermoproteus tenax | |
| 0.734 | - |
D-fructose | at 50°C, pH 7.5 | Thermoproteus tenax | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | strict requirement for divalent metal ions | Thermoproteus tenax | |
| Mn2+ | strict requirement for divalent metal ions | Thermoproteus tenax | |
| Mn2+ | Mg2+ can be partially replaced by Mn2+ | Thermoproteus tenax | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32543 | - |
1 * 32543, calculated from the amino acid sequence, x * 33000, SDS-PAGE | Thermoproteus tenax |
| 33000 | - |
1 * 32543, calculated from the amino acid sequence, x * 33000, SDS-PAGE | Thermoproteus tenax |
| 40000 | - |
gel filtration | Thermoproteus tenax |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + ATP | Thermoproteus tenax | carbohydrate metabolism | D-glucose 6-phosphate + ADP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoproteus tenax | Q7ZAH1 | Kra 1, i.e. DSM 2078 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant hexokinase, expressed in Escherichia coli | Thermoproteus tenax |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Thermoproteus tenax |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-deoxy-D-glucose | - |
Thermoproteus tenax | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
| D-fructose + ATP | - |
Thermoproteus tenax | ADP + D-fructose 6-phosphate | - |
? | |
| D-glucose + ATP | preferred specificity for glucose | Thermoproteus tenax | ADP + D-glucose 6-phosphate | - |
? | |
| D-glucose + ATP | carbohydrate metabolism | Thermoproteus tenax | D-glucose 6-phosphate + ADP | - |
? | |
| D-mannose + ATP | - |
Thermoproteus tenax | ADP + D-mannose 6-phosphate | - |
? | |
| GTP + D-glucose | 18% of the activity with ATP | Thermoproteus tenax | GDP + D-glucose 6-phosphate | - |
? | |
| ITP + D-glucose | 40% of the activity with ATP | Thermoproteus tenax | IDP + D-glucose 6-phosphate | - |
? | |
| additional information | not: ADP | Thermoproteus tenax | ? | - |
? | |
| additional information | not: ribose, xylose, phosphorylated hexoses, UDP, polyphosphates | Thermoproteus tenax | ? | - |
? | |
| additional information | not: D-galactose | Thermoproteus tenax | ? | - |
? | |
| additional information | not: CTP | Thermoproteus tenax | ? | - |
? | |
| UTP + D-glucose | 17% of the activity with ATP | Thermoproteus tenax | UDP + D-glucose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32543, calculated from the amino acid sequence, x * 33000, SDS-PAGE | Thermoproteus tenax |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Thermoproteus tenax |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Thermoproteus tenax |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Thermoproteus tenax | |
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