General Stability | Organism |
---|---|
D-glucose or D-fructose, 5 mM, stabilizes | Oryctolagus cuniculus |
glycerol, 9-15% v/v, stabilizes | Oryctolagus cuniculus |
sulfhydryl protecting agents, e.g. DTT or 2-mercaptoethanol, required for optimum stability | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
D-glucose | at 37°C, pH 8.1 | Oryctolagus cuniculus | |
0.1 | - |
D-mannose | at 37°C, pH 8.1 | Oryctolagus cuniculus | |
0.4 | - |
D-glucosamine | at 37°C, pH 8.1 | Oryctolagus cuniculus | |
0.62 | - |
ATP | MgATP2-, at 37°C, pH 8.1 | Oryctolagus cuniculus | |
2 | - |
2-deoxy-D-glucose | at 37°C, pH 8.1 | Oryctolagus cuniculus | |
4.5 | - |
D-fructose | at 37°C, pH 8.1 | Oryctolagus cuniculus | |
10 | - |
ITP | MgITP2-, at 37°C, pH 8.1 | Oryctolagus cuniculus | |
30 | - |
UTP | MgUTP2-, at 37°C, pH 8.1 | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Oryctolagus cuniculus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
sucrose density gradient sedimentation | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
717fold | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
165 | - |
at 37°C | Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
4°C or -20°C, 5 mM sodium potassium phosphate buffer, pH 7.5, 9% v/v glycerol, 5 mM glucose, 3 mM mercaptoethanol, several days, stable | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-D-glucose | 54% of the activity with glucose | Oryctolagus cuniculus | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
ATP + 2-deoxy-D-glucose | 84% of the activity with glucose | Oryctolagus cuniculus | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
D-fructose + ATP | 110% of the activity with glucose | Oryctolagus cuniculus | ADP + D-fructose 6-phosphate | - |
? | |
D-glucosamine + ATP | - |
Oryctolagus cuniculus | ADP + D-glucosamine 6-phosphate | - |
? | |
D-glucose + ATP | - |
Oryctolagus cuniculus | ADP + D-glucose 6-phosphate | - |
? | |
D-mannose + ATP | 82% of the activity with glucose | Oryctolagus cuniculus | ADP + D-mannose 6-phosphate | - |
? | |
ITP + D-glucose | 12% of the activity with ATP | Oryctolagus cuniculus | IDP + D-glucose 6-phosphate | - |
? | |
ITP + D-glucose | MgITP | Oryctolagus cuniculus | IDP + D-glucose 6-phosphate | - |
? | |
additional information | not: N-acetyl-D-glucosamine | Oryctolagus cuniculus | ? | - |
? | |
additional information | not: D-galactose | Oryctolagus cuniculus | ? | - |
? | |
additional information | not: CTP | Oryctolagus cuniculus | ? | - |
? | |
UTP + D-glucose | 14% of the activity with ATP | Oryctolagus cuniculus | UDP + D-glucose 6-phosphate | - |
? | |
UTP + D-glucose | MgUTP | Oryctolagus cuniculus | UDP + D-glucose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 100000, SDS-PAGE | Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | in glycyl-glycine buffer | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Oryctolagus cuniculus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Oryctolagus cuniculus | isoelectric focusing | 6.8 | 6.3 |