General Stability | Organism |
---|---|
D-glucose protects against inactivation at higher temperatures | Homo sapiens |
glycerol, 9-15% v/v, stabilizes | Homo sapiens |
sulfhydryl protecting agents, e.g. DTT or 2-mercaptoethanol, required for optimum stability | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose 1,6-diphosphate | competitive to MgATP2-, inhibition is partially relieved by phosphate | Homo sapiens | |
D-glucose 6-phosphate | competitive to MgATP2-; inhibition is partially relieved by phosphate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | 0.048 | D-glucose | hexokinases Ia, Ib and Ic, at 37°C, pH 7.2 | Homo sapiens | |
0.07 | 0.1 | D-mannose | hexokinases Ia, Ib and Ic, at 37°C, pH 7.2 | Homo sapiens | |
0.5 | - |
D-glucosamine | hexokinases Ib and Ic, at 37°C, pH 7.2 | Homo sapiens | |
0.56 | - |
2-deoxy-D-glucose | hexokinase Ib, at 37°C, pH 7.2 | Homo sapiens | |
0.58 | 0.62 | ATP | MgATP2-, hexokinases Ia, Ib and Ic, at 37°C, pH 7.2 | Homo sapiens | |
0.77 | - |
2-deoxy-D-glucose | hexokinase Ia, at 37°C, pH 7.2 | Homo sapiens | |
1.16 | - |
2-deoxy-D-glucose | hexokinase Ic, at 37°C, pH 7.2 | Homo sapiens | |
1.5 | - |
D-glucosamine | hexokinase Ia, at 37°C, pH 7.2 | Homo sapiens | |
10 | 13 | D-fructose | hexokinases Ia, Ib and Ic, at 37°C, pH 7.2 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | liver hexokinase | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
sucrose density gradient sedimentation | Homo sapiens |
100000 | - |
hexokinases Ia, Ib and Ic | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
multiple forms: Ia, Ib, Ic | - |
Purification (Comment) | Organism |
---|---|
partial, from adult and fetal erythrocytes, 1000-2000fold | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | 3 major forms: hexokinase Ia corresponds to type I from human liver, hexokinase Ib is the predominant form in fetal erythrocytes, hexokinase Ic, cell age dependence of the isoenzymic pattern | Homo sapiens | - |
liver | 4 isoenzymes, lower level of hexokinase IV than in rat liver | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.55 | 1.95 | hexokinases Ia, Ib and Ic from adult erythrocytes, at 37°C, pH 7.2 | Homo sapiens |
1.43 | 2.22 | hexokinases Ia, Ib and Ic from umbilical cord blood, at 37°C, pH 7.2 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-D-glucose | Hex Ia: 95% of the activity with glucose, Hex Ib: 99% of the activity with glucose, Hex Ic: 74% of the activity with glucose | Homo sapiens | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
CTP + D-glucose | - |
Homo sapiens | CDP + D-glucose 6-phosphate | - |
? | |
D-fructose + ATP | Hex Ia: 100% of the activity with glucose, Hex Ib: 120% of the activity with glucose, Hex Ic: 90% of the activity with glucose | Homo sapiens | ADP + D-fructose 6-phosphate | - |
? | |
D-glucosamine + ATP | Hex Ia and Ib: 47% of the activity with glucose, Hex Ic: 21% of the activity with glucose | Homo sapiens | ADP + D-glucosamine 6-phosphate | - |
? | |
D-glucose + ATP | - |
Homo sapiens | ADP + D-glucose 6-phosphate | - |
? | |
D-mannose + ATP | Hex Ia: 78% of the activity with glucose, Hex Ib: 83% of the activity with glucose, Hex Ic: 85% of the activity with glucose | Homo sapiens | ADP + D-mannose 6-phosphate | - |
? | |
ITP + D-glucose | 13.5-14% of the activity with ATP | Homo sapiens | IDP + D-glucose 6-phosphate | - |
? | |
ITP + D-glucose | MgITP | Homo sapiens | IDP + D-glucose 6-phosphate | - |
? | |
additional information | not: UTP | Homo sapiens | ? | - |
? | |
additional information | not: N-acetyl-D-glucosamine | Homo sapiens | ? | - |
? | |
additional information | not: D-galactose | Homo sapiens | ? | - |
? | |
additional information | not: GTP | Homo sapiens | ? | - |
? | |
additional information | not: CTP | Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
glucose protects against inactivation at higher temperatures | Homo sapiens |
44 | - |
5 mM Tris-HCl, pH 7.2, 9% v/v glycerol, 3 mM 2-mercaptoethanol, 20 min, about 30% loss of activity, glucose protects | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | activity increases more than 40% over the range of pH 7-8, after which the activity remains constant until pH 9 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | 15 | glucose 6-phosphate | hexokinase Ia, Ib and Ic, at 37°C, pH 7.2 | Homo sapiens | |
21 | 22 | D-glucose 1,6-diphosphate | hexokinase Ia, Ib and Ic, at 37°C, pH 7.2 | Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | hexokinase Ic, isoelectric focusing | - |
5.35 |
Homo sapiens | hexokinase Ib, isoelectric focusing | - |
5.5 |
Homo sapiens | hexokinase Ia, isoelectric focusing | - |
5.7 |