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Literature summary for 2.7.1.1 extracted from

  • Stocchi, V.; Magnani, M.; Canestrari, F.; Dacha, M.; Fornaini, G.
    Multiple forms of human red blood cell hexokinase. Preparation, characterization, and age dependence (1982), J. Biol. Chem., 257, 2357-2364.
    View publication on PubMed
Search for more literature for 2.7.1.1

General Stability

General Stability Organism
D-glucose protects against inactivation at higher temperatures Homo sapiens
glycerol, 9-15% v/v, stabilizes Homo sapiens
sulfhydryl protecting agents, e.g. DTT or 2-mercaptoethanol, required for optimum stability Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
D-glucose 1,6-diphosphate competitive to MgATP2-, inhibition is partially relieved by phosphate Homo sapiens
D-glucose 6-phosphate competitive to MgATP2-; inhibition is partially relieved by phosphate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046 0.048 D-glucose hexokinases Ia, Ib and Ic, at 37°C, pH 7.2 Homo sapiens
0.07 0.1 D-mannose hexokinases Ia, Ib and Ic, at 37°C, pH 7.2 Homo sapiens
0.5
-
 D-glucosamine hexokinases Ib and Ic, at 37°C, pH 7.2 Homo sapiens
0.56
-
 2-deoxy-D-glucose hexokinase Ib, at 37°C, pH 7.2 Homo sapiens
0.58 0.62 ATP MgATP2-, hexokinases Ia, Ib and Ic, at 37°C, pH 7.2 Homo sapiens
0.77
-
 2-deoxy-D-glucose hexokinase Ia, at 37°C, pH 7.2 Homo sapiens
1.16
-
 2-deoxy-D-glucose hexokinase Ic, at 37°C, pH 7.2 Homo sapiens
1.5
-
 D-glucosamine hexokinase Ia, at 37°C, pH 7.2 Homo sapiens
10 13 D-fructose hexokinases Ia, Ib and Ic, at 37°C, pH 7.2 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble liver hexokinase Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 sucrose density gradient sedimentation Homo sapiens
100000
-
 hexokinases Ia, Ib and Ic Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 multiple forms: Ia, Ib, Ic
-

Purification (Commentary)

Purification (Comment) Organism
partial, from adult and fetal erythrocytes, 1000-2000fold Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte 3 major forms: hexokinase Ia corresponds to type I from human liver, hexokinase Ib is the predominant form in fetal erythrocytes, hexokinase Ic, cell age dependence of the isoenzymic pattern Homo sapiens
-
liver 4 isoenzymes, lower level of hexokinase IV than in rat liver Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.55 1.95 hexokinases Ia, Ib and Ic from adult erythrocytes, at 37°C, pH 7.2 Homo sapiens
1.43 2.22 hexokinases Ia, Ib and Ic from umbilical cord blood, at 37°C, pH 7.2 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-D-glucose Hex Ia: 95% of the activity with glucose, Hex Ib: 99% of the activity with glucose, Hex Ic: 74% of the activity with glucose Homo sapiens ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
CTP + D-glucose
-
 Homo sapiens CDP + D-glucose 6-phosphate
-
 ?
D-fructose + ATP Hex Ia: 100% of the activity with glucose, Hex Ib: 120% of the activity with glucose, Hex Ic: 90% of the activity with glucose Homo sapiens ADP + D-fructose 6-phosphate
-
 ?
D-glucosamine + ATP Hex Ia and Ib: 47% of the activity with glucose, Hex Ic: 21% of the activity with glucose Homo sapiens ADP + D-glucosamine 6-phosphate
-
 ?
D-glucose + ATP
-
 Homo sapiens ADP + D-glucose 6-phosphate
-
 ?
D-mannose + ATP Hex Ia: 78% of the activity with glucose, Hex Ib: 83% of the activity with glucose, Hex Ic: 85% of the activity with glucose Homo sapiens ADP + D-mannose 6-phosphate
-
 ?
ITP + D-glucose 13.5-14% of the activity with ATP Homo sapiens IDP + D-glucose 6-phosphate
-
 ?
ITP + D-glucose MgITP Homo sapiens IDP + D-glucose 6-phosphate
-
 ?
additional information not: UTP Homo sapiens ?
-
 ?
additional information not: N-acetyl-D-glucosamine Homo sapiens ?
-
 ?
additional information not: D-galactose Homo sapiens ?
-
 ?
additional information not: GTP Homo sapiens ?
-
 ?
additional information not: CTP Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 glucose protects against inactivation at higher temperatures Homo sapiens
44
-
 5 mM Tris-HCl, pH 7.2, 9% v/v glycerol, 3 mM 2-mercaptoethanol, 20 min, about 30% loss of activity, glucose protects Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7 9 activity increases more than 40% over the range of pH 7-8, after which the activity remains constant until pH 9 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
13 15 glucose 6-phosphate hexokinase Ia, Ib and Ic, at 37°C, pH 7.2 Homo sapiens
21 22 D-glucose 1,6-diphosphate hexokinase Ia, Ib and Ic, at 37°C, pH 7.2 Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens hexokinase Ic, isoelectric focusing
-
 5.35
Homo sapiens hexokinase Ib, isoelectric focusing
-
 5.5
Homo sapiens hexokinase Ia, isoelectric focusing
-
 5.7