Cloned (Comment) | Organism |
---|---|
HXK I and HXK II hexokinase genes are cloned | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
isoenzymes PI and PII, large crystals in the native and complexed form with glucose | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
1 * 54000, both isoenzymes PI and PII, SDS-PAGE | Saccharomyces cerevisiae |
55000 | 60000 | both isoenzymes PI and PII, FPLC gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
overproducing transformed strain WAY.10-1C, isoenzymes PI and PII | - |
Purification (Comment) | Organism |
---|---|
from overproducing strain | Saccharomyces cerevisiae |
isoenzymes PI and PII | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, purified isoenzymes PI and PII, 25 mM piperazine-HCl, pH 6.5, 50% glycerol, at least several months, minor loss of activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + ATP | - |
Saccharomyces cerevisiae | ADP + D-fructose 6-phosphate | - |
? | |
D-glucose + ATP | - |
Saccharomyces cerevisiae | ADP + D-glucose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 54000, both isoenzymes PI and PII, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |