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Literature summary for 2.7.1.1 extracted from
- Simultaneous purification of hexokinase, class-I fructose-bisphosphate aldolase, triosephosphate isomerase and phosphoglycerate kinase from Trypanosoma brucei (1984), Eur. J. Biochem., 144, 475-483.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | hexokinase may be an excellent target for the development of a rational chemotherapy against the parasite | Trypanosoma brucei |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| glycosome | - |
Trypanosoma brucei | 20015 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, SDS-PAGE | Trypanosoma brucei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + ATP | Trypanosoma brucei | rate-limiting step in the glycolysis | D-glucose 6-phosphate + ADP | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | - |
bloodstream forms, stock 427 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 260fold | Trypanosoma brucei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
in purified glycosomes, at 25°C, pH 7.6 | Trypanosoma brucei |
| 190 | - |
pure enzyme, at 25°C, pH 7.6 | Trypanosoma brucei |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, extensive dialysis of purified enzyme against 0.1 M Tris/HCl, pH 7.6, 1 mM dithiothreitol, 1 mM EDTA, 1 mM sodium azide, 0.2 M ammonium sulfate, stable | Trypanosoma brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + ATP | - |
Trypanosoma brucei | ADP + D-glucose 6-phosphate | - |
? | |
| D-glucose + ATP | rate-limiting step in the glycolysis | Trypanosoma brucei | D-glucose 6-phosphate + ADP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, SDS-PAGE | Trypanosoma brucei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Trypanosoma brucei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Trypanosoma brucei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Trypanosoma brucei | |
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