Cloned (Comment) | Organism |
---|---|
gene sav3, DNA and amino acid sequence deterination and analysis, SAV3 has a localized and dynamic expression pattern | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + pyruvate | Arabidopsis thaliana | SAV3 is specific for L-Trp | indole-3-pyruvate + L-alanine | - |
? | |
L-tryptophan + pyruvate | Arabidopsis thaliana Col-0 | SAV3 is specific for L-Trp | indole-3-pyruvate + L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9S7N2 | - |
- |
Arabidopsis thaliana Col-0 | Q9S7N2 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hypocotyl | - |
Arabidopsis thaliana | - |
leaf | high expression level of SAV3 | Arabidopsis thaliana | - |
additional information | SAV3 has a localized and dynamic expression pattern | Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + pyruvate | SAV3 is specific for L-Trp | Arabidopsis thaliana | indole-3-pyruvate + L-alanine | - |
? | |
L-tryptophan + pyruvate | L-Trp is the preferred substrate | Arabidopsis thaliana | indole-3-pyruvate + L-alanine | - |
? | |
L-tryptophan + pyruvate | SAV3 is specific for L-Trp | Arabidopsis thaliana Col-0 | indole-3-pyruvate + L-alanine | - |
? | |
L-tryptophan + pyruvate | L-Trp is the preferred substrate | Arabidopsis thaliana Col-0 | indole-3-pyruvate + L-alanine | - |
? | |
additional information | SAV3 also uses L-Phe, Tyr, Leu, Ala, Met and Gln as substrates in vitro | Arabidopsis thaliana | ? | - |
? | |
additional information | SAV3 also uses L-Phe, Tyr, Leu, Ala, Met and Gln as substrates in vitro | Arabidopsis thaliana Col-0 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | SAV3 contains a C-terminal alliinase/aminotransferase domain | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
SAV3 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | SAV3 mutants are unable to elongate in simulated shade light, phenotypes, overview. Other indole acetic acid biosynthetic pathways cannot compensate for the loss of the SAV3-dependent pathway. sav3 mutants have reduced auxin levels and a diminished auxin response, and sav3 mutants exhibit shorter hypocotyls than WT when grown in simulated shade and partially suppress the constitutive shade avoidance phenotype of a phyB null mutant, sav3-1 plants are shorter and have reduced leaf hyponasty as compared to wild-type plants. The sav3-1 mutant fails to induce shade avoidance syndrome, SAS, in a controlled environment typically used to detect PHYB-mediated SAS responses in light-grown plants | Arabidopsis thaliana |
additional information | SAV3 in silico docking using the crystal structure obtained from SAV3 crystals soaked in L-Phe and co-crystallized with pyridoxal 5'-phosphate, for analyzing substrate and cofactor pecificities, overview | Arabidopsis thaliana |
physiological function | SAV3 is an L-Trp aminotransferase involved in indole 3-acetic acid biosynthesis. SAV3 catalyzes the formation of indole-3-pyruvic acid from L-tryptophan as first step in an auxin biosynthetic pathway. Feedback regulation on SAV3 expression by shade | Arabidopsis thaliana |