Literature summary for 2.6.1.93 extracted from

Huang, F.; Spiteller, D.; Koorbanally, N.A.; Li, Y.; Llewellyn, N.M.; Spencer, J.B.
Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin (2007), ChemBioChem, 8, 283-288.

Search for more literature for 2.6.1.93

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Niallia circulans
expressed in Escherichia coli BL21(DE3) cells	Streptomyces fradiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48060	-	Neo-18, calculated from amino acid sequence	Streptomyces fradiae
48060	-	Neo-18, electrospray ionization mass spectrometry	Streptomyces fradiae
52070	-	BtrB, calculated from amino acid sequence	Niallia circulans
52070	-	BtrB, electrospray ionization mass spectrometry	Niallia circulans

Organism

Organism	UniProt	Comment	Textmining
Niallia circulans	-	-	-
Niallia circulans B3312	-	-	-
Streptomyces fradiae	-	-	-
Streptomyces fradiae NCIMB 8233	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-NTA resin column chromatography	Niallia circulans
Ni2+-NTA resin column chromatography	Streptomyces fradiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6'-dehydroparomamine + L-glutamate	-	Niallia circulans	neamine + 2-oxoglutarate	-	?
6'-dehydroparomamine + L-glutamate	-	Streptomyces fradiae	neamine + 2-oxoglutarate	-	?
6'-dehydroparomamine + L-glutamate	-	Streptomyces fradiae NCIMB 8233	neamine + 2-oxoglutarate	-	?
6'-dehydroparomamine + L-glutamate	-	Niallia circulans B3312	neamine + 2-oxoglutarate	-	?

Synonyms

Synonyms	Comment	Organism
6'-oxoglucosaminyl:L-glutamate aminotransferase	-	Niallia circulans
6'-oxoglucosaminyl:L-glutamate aminotransferase	-	Streptomyces fradiae
btrB	-	Niallia circulans
Neo-18	-	Streptomyces fradiae

General Information

General Information	Comment	Organism
metabolism	BtrB is involved in neamine biosynthesis	Niallia circulans
metabolism	Neo-18 is involved in neamine biosynthesis	Streptomyces fradiae

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Release 2023.1 (January 2023)