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Literature summary for 2.6.1.85 extracted from

  • Culbertson, J.E.; Toney, M.D.
    Expression and characterization of PhzE from P. aeruginosa PAO1: aminodeoxyisochorismate synthase involved in pyocyanin and phenazine-1-carboxylate production (2013), Biochim. Biophys. Acta, 1834, 240-246.
    View publication on PubMed
Search for more literature for 2.6.1.85

Inhibitors

Inhibitors Comment Organism Structure
1-hydroxyphenazine inhibitory above 0.200 mM Pseudomonas aeruginosa
additional information enzyme is not regulated by feedback inhibition. Little or no inhibition up to 1 mM: glyphosate, pyridoxamine 5'-phosphate, pyridoxal 5'-phosphate, and 3-hydroxypyridine-4-carboxylic acid Pseudomonas aeruginosa
phosphate phosphate buffer competitively inhibits PhzE, about 20% inhibition with 100 mM potassium phosphate at pH 7.0 Pseudomonas aeruginosa
pyocyanin inhibitory above 0.250 mM Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 chorismate cosubstrate L-glutamine, pH 7.0, 25°C Pseudomonas aeruginosa
0.216
-
 chorismate cosubstrate L-glutamine, pH 7.0, 25°C Pseudomonas aeruginosa
11
-
 L-glutamine pH 7.0, 25°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Pseudomonas aeruginosa
Mg2+ required, Km value 0.29 mM Pseudomonas aeruginosa
Mn2+
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71251
-
-
 Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q7DC81
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate + L-glutamine
-
 Pseudomonas aeruginosa L-glutamate + 4-amino-4-deoxychorismate
-
 ?
chorismate + NH3
-
 Pseudomonas aeruginosa 4-amino-4-deoxychorismate + H2O
-
 ?
additional information isoform PhzE catalyzes the addition of ammonia to C2 of chorismate, as does anthranilate synthase, yet unlike anthranilate synthase it does not catalyze elimination of pyruvate from enzyme-bound aminodeoxyisochorismate. Reaction follows a random kinetic mechanism, enzyme PhzE can use NH4+ as an alternative nucleophile. PhzE shows no lyase activity Pseudomonas aeruginosa ?
-
 ?

Subunits

Subunits Comment Organism
? x * 71274, calculated, x * 71251, MALDI-TOF, His-tagged recombinant protein Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
PhzE
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30
-
 Pseudomonas aeruginosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45
-
 20% of maximum activtiy Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.16
-
 chorismate cosubstrate NH3, pH 7.0, 25°C Pseudomonas aeruginosa
2.2
-
 chorismate cosubstrate L-glutamine, pH 7.0, 25°C Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Pseudomonas aeruginosa