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Literature summary for 2.6.1.84 extracted from

  • Yang, Z.; Lu, C.D.
    Characterization of an arginine:pyruvate transaminase in arginine catabolism of Pseudomonas aeruginosa PAO1 (2007), J. Bacteriol., 189, 3954-3959.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.84

Cloned(Commentary)

Cloned (Comment) Organism
gene aruH, overexpression of soluble His-tagged enzyme in Escherichia coli Pseudomonas aeruginosa
overexpressed in Escherichia coli Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
borate/NaOH buffer strong inhibition at pH 9.0 Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Pseudomonas aeruginosa
additional information
-
 additional information catalytic efficiency kcat/KM 24.1 mM/s for pyruvate and 2.8 mM/s for arginine Pseudomonas aeruginosa
1.6
-
 pyruvate pH 9.0, 37°C Pseudomonas aeruginosa
1.6
-
 pyruvate pH 9.0, 37°C, coupled reaction with NAD+ and L-alanine dehydrogenase Pseudomonas aeruginosa
2.8
-
 L-arginine pH 9.0, 37°C Pseudomonas aeruginosa
13.9
-
 L-arginine pH 9.0, 37°C, coupled reaction with NAD+ and L-alanine dehydrogenase Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 2 * 43000, recombinant enzyme, SDS-PAGE Pseudomonas aeruginosa
43000
-
 2 * 43000, SDS-PAGE, His-tagged Pseudomonas aeruginosa
79300
-
 recombinant enzyme, gel filtration Pseudomonas aeruginosa
79300
-
 native enzyme, gel filtration, apparent mass, indicating that His-tagged AruH is a homodimer Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine + pyruvate Pseudomonas aeruginosa AruH catalyzes the first step of the arginine transaminase pathway, representing one of the multiple pathways for L-arginine catabolism 5-guanidino-2-oxopentanoate + L-alanine
-
 ?
L-arginine + pyruvate Pseudomonas aeruginosa AruH catalyzes the first step in the arginine transaminase pathway, converting the substrates L-arginine and pyruvate into 2-ketoarginine and L-alanine, AruH may have a broader physiological function in amino acid catabolism 5-guanidino-2-oxopentanoate + L-alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa
-
 gene aruH
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, to homogeneity, using HisTrap HP kit, anion exchange chromatography Mono Q Pseudomonas aeruginosa
recombinant His-tagged AruH from Escherichia coli to homogeneity by affinity and anion exchange chromatography Pseudomonas aeruginosa

Reaction

Reaction Comment Organism Reaction ID
L-arginine + pyruvate = 5-guanidino-2-oxopentanoate + L-alanine ping-pong kinetic mechanism Pseudomonas aeruginosa
a
L-arginine + pyruvate = 5-guanidino-2-oxopentanoate + L-alanine AruH catalyzes the first step of the arginine transaminase pathway, representing one of the multiple pathways for L-arginine catabolism Pseudomonas aeruginosa
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 a coupled reaction assay with NAD+ and L-alanine dehydrogenase Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + pyruvate
-
 Pseudomonas aeruginosa 5-guanidino-2-oxopentanoate + L-alanine product identification by mass spectrometry ?
L-arginine + pyruvate AruH catalyzes the first step of the arginine transaminase pathway, representing one of the multiple pathways for L-arginine catabolism Pseudomonas aeruginosa 5-guanidino-2-oxopentanoate + L-alanine
-
 ?
L-arginine + pyruvate broad substrate specificity, preference in decreasing order: L-arginine, L-lysine, L-methionine, L-leucine, L-ornithine, L-glutamine Pseudomonas aeruginosa 5-guanidino-2-oxopentanoate + L-alanine detected by HPLC and mass spectrometry ?
L-arginine + pyruvate AruH catalyzes the first step in the arginine transaminase pathway, converting the substrates L-arginine and pyruvate into 2-ketoarginine and L-alanine, AruH may have a broader physiological function in amino acid catabolism Pseudomonas aeruginosa 5-guanidino-2-oxopentanoate + L-alanine
-
 ?
L-glutamine + pyruvate broad substrate specificity, preference in decreasing order: L-arginine, L-lysine, L-methionine, L-leucine, L-ornithine, L-glutamine Pseudomonas aeruginosa 2-oxoglutaramate + L-alanine
-
 ?
L-glutamine + pyruvate
-
 Pseudomonas aeruginosa ?
-
 ?
L-leucine + pyruvate
-
 Pseudomonas aeruginosa 4-methyl-2-oxopentanoate + L-alanine
-
 ?
L-leucine + pyruvate broad substrate specificity, preference in decreasing order: L-arginine, L-lysine, L-methionine, L-leucine, L-ornithine, L-glutamine Pseudomonas aeruginosa 4-methyl-2-oxopentanoate + L-alanine
-
 ?
L-lysine + pyruvate broad substrate specificity, preference in decreasing order: L-arginine, L-lysine, L-methionine, L-leucine, L-ornithine, L-glutamine Pseudomonas aeruginosa DELTA1-piperidine-2-carboxylate + L-alanine mass spectrometry analysis, implies that AruH may have broader physiological functions in amino acid catabolism ?
L-lysine + pyruvate
-
 Pseudomonas aeruginosa DELTA1-piperideine-2-carboxylate + L-alanine product identification by mass spectrometry ?
L-methionine + pyruvate broad substrate specificity, preference in decreasing order: L-arginine, L-lysine, L-methionine, L-leucine, L-ornithine, L-glutamine Pseudomonas aeruginosa 4-methylthio-2-oxobutanoate + L-alanine
-
 ?
L-methionine + pyruvate
-
 Pseudomonas aeruginosa 4-methylthio-2-oxo-butanoate + L-alanine
-
 ?
L-ornithine + pyruvate broad substrate specificity, preference in decreasing order: L-arginine, L-lysine, L-methionine, L-leucine, L-ornithine, L-glutamine Pseudomonas aeruginosa 5-amino-2-oxopentanoate + L-alanine
-
 ?
L-ornithine + pyruvate
-
 Pseudomonas aeruginosa ?
-
 ?
additional information broad substrate specificity in an descending order of preference: Arg, Lys, Met, Leu, Orn, Gln Pseudomonas aeruginosa ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 43000, recombinant enzyme, SDS-PAGE Pseudomonas aeruginosa
homodimer 2 * 43000, SDS-PAGE, His-tagged Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
arginine:pyruvate transaminase
-
 Pseudomonas aeruginosa
AruH
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
 Pseudomonas aeruginosa
42
-
 sharp inactivation above Pseudomonas aeruginosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 42 the enzyme is prone to sharp thermal inactivation above this temperature Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information catalytic efficiency kcat/KM 24.1 mM/s for pyruvate and 2.8 mM/s for arginine Pseudomonas aeruginosa
38.6
-
 L-arginine pH 9.0, 37°C Pseudomonas aeruginosa
38.6
-
 pyruvate calculated, pH 9.0, 37°C, coupled reaction with NAD+ and L-alanine dehydrogenase Pseudomonas aeruginosa
38.6
-
 L-arginine calculated, pH 9.0, 37°C, coupled reaction with NAD+ and L-alanine dehydrogenase Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Pseudomonas aeruginosa
9
-
 in borate/NaOH buffer Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.5 activity range Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Pseudomonas aeruginosa