Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis | Bilophila wadsworthia |
General Stability | Organism |
---|---|
glycerol stabilizes | Bilophila wadsworthia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.82 | - |
pyruvate | pH 9.0, 35°C | Bilophila wadsworthia | |
7.1 | - |
taurine | pH 9.0, 35°C | Bilophila wadsworthia | |
8.1 | - |
Hypotaurine | pH 9.0, 35°C | Bilophila wadsworthia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
4 * 51000, SDS-PAGE | Bilophila wadsworthia |
197000 | - |
gel filtration | Bilophila wadsworthia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
taurine + pyruvate | Bilophila wadsworthia | initial step in anaerobic taurine degradation | L-alanine + 2-sulfoacetaldehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bilophila wadsworthia | Q9APM5 | RZATAU | - |
Bilophila wadsworthia RZATAU | Q9APM5 | RZATAU | - |
Purification (Comment) | Organism |
---|---|
buffer MOPS, pH 6.5, apparent homogeneity | Bilophila wadsworthia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity depends strongly on growth substrate | Bilophila wadsworthia |
1.02 | - |
taurine/formate-grown cells | Bilophila wadsworthia |
2.22 | - |
pyruvate-grown cells | Bilophila wadsworthia |
35.3 | - |
purified enzyme | Bilophila wadsworthia |
Storage Stability | Organism |
---|---|
4°C or 22°C, sulfonate buffers MES or MOPS, pH range 6.0-7.0, 22 h, stable | Bilophila wadsworthia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-aminopropanesulfonate + pyruvate | low activity | Bilophila wadsworthia | ? | - |
? | |
3-aminopropanesulfonate + pyruvate | purified enzyme | Bilophila wadsworthia | ? | - |
? | |
beta-alanine + pyruvate | purified enzyme | Bilophila wadsworthia | ? | - |
? | |
cysteine + pyruvate | low activity | Bilophila wadsworthia | ? | - |
? | |
cysteine + pyruvate | purified enzyme | Bilophila wadsworthia | ? | - |
? | |
hypotaurine + pyruvate | i.e. 2-aminoethanesulfinate | Bilophila wadsworthia | ? | - |
? | |
hypotaurine + pyruvate | purified enzyme | Bilophila wadsworthia | ? | - |
? | |
hypotaurine + pyruvate | i.e. 2-aminoethanesulfinate | Bilophila wadsworthia RZATAU | ? | - |
? | |
hypotaurine + pyruvate | purified enzyme | Bilophila wadsworthia RZATAU | ? | - |
? | |
additional information | no activity with 2-oxoglutarate and phenylpyruvate as amino group acceptor | Bilophila wadsworthia | ? | - |
? | |
additional information | cysteate, glutamate and 2-aminoethanephosphonate are poor amino group donor substrates | Bilophila wadsworthia | ? | - |
? | |
additional information | no activity with 2-oxoglutarate and phenylpyruvate as amino group acceptor | Bilophila wadsworthia RZATAU | ? | - |
? | |
additional information | cysteate, glutamate and 2-aminoethanephosphonate are poor amino group donor substrates | Bilophila wadsworthia RZATAU | ? | - |
? | |
taurine + 2-oxobutyrate | - |
Bilophila wadsworthia | 2-sulfoacetaldehyde + 2-aminobutyrate | - |
? | |
taurine + oxaloacetate | - |
Bilophila wadsworthia | ? | - |
? | |
taurine + pyruvate | i.e. 2-aminoethanesulfonate | Bilophila wadsworthia | 2-sulfoacetaldehyde + L-alanine | - |
r | |
taurine + pyruvate | i.e. 2-aminoethanesulfonate | Bilophila wadsworthia RZATAU | 2-sulfoacetaldehyde + L-alanine | - |
r | |
taurine + pyruvate | initial step in anaerobic taurine degradation | Bilophila wadsworthia | L-alanine + 2-sulfoacetaldehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | formation of large aggregates depends on storage conditions, freezing at -20°C, or rapid freezing in liquid nitrogen with storage at -20°C increases the 350 kDa form, addition of glycerol decreases the aggregation, a dimer of 95 kDa is preferably formed during storage at 4°C | Bilophila wadsworthia |
tetramer | 4 * 51000, SDS-PAGE | Bilophila wadsworthia |
Synonyms | Comment | Organism |
---|---|---|
tPA | - |
Bilophila wadsworthia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Bilophila wadsworthia |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | broad optimum | Bilophila wadsworthia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Bilophila wadsworthia |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme is unstable in buffers with neutral or alkaline pH value, except for HEPES at pH 7.0 | Bilophila wadsworthia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | activating | Bilophila wadsworthia | |
pyridoxal 5'-phosphate | pyridoxyl 5'-phosphate is not essentially required | Bilophila wadsworthia |