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Literature summary for 2.6.1.77 extracted from

  • Laue, H.; Cook, A.M.
    Biochemical and molecular characterization of taurine:pyruvate transaminase from the anaerobe Bilophila wadsworthia (2000), Eur. J. Biochem., 267, 6841-6848.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis Bilophila wadsworthia

General Stability

General Stability Organism
glycerol stabilizes Bilophila wadsworthia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.82
-
pyruvate pH 9.0, 35°C Bilophila wadsworthia
7.1
-
taurine pH 9.0, 35°C Bilophila wadsworthia
8.1
-
Hypotaurine pH 9.0, 35°C Bilophila wadsworthia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
4 * 51000, SDS-PAGE Bilophila wadsworthia
197000
-
gel filtration Bilophila wadsworthia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
taurine + pyruvate Bilophila wadsworthia initial step in anaerobic taurine degradation L-alanine + 2-sulfoacetaldehyde
-
?

Organism

Organism UniProt Comment Textmining
Bilophila wadsworthia Q9APM5 RZATAU
-
Bilophila wadsworthia RZATAU Q9APM5 RZATAU
-

Purification (Commentary)

Purification (Comment) Organism
buffer MOPS, pH 6.5, apparent homogeneity Bilophila wadsworthia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity depends strongly on growth substrate Bilophila wadsworthia
1.02
-
taurine/formate-grown cells Bilophila wadsworthia
2.22
-
pyruvate-grown cells Bilophila wadsworthia
35.3
-
purified enzyme Bilophila wadsworthia

Storage Stability

Storage Stability Organism
4°C or 22°C, sulfonate buffers MES or MOPS, pH range 6.0-7.0, 22 h, stable Bilophila wadsworthia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-aminopropanesulfonate + pyruvate low activity Bilophila wadsworthia ?
-
?
3-aminopropanesulfonate + pyruvate purified enzyme Bilophila wadsworthia ?
-
?
beta-alanine + pyruvate purified enzyme Bilophila wadsworthia ?
-
?
cysteine + pyruvate low activity Bilophila wadsworthia ?
-
?
cysteine + pyruvate purified enzyme Bilophila wadsworthia ?
-
?
hypotaurine + pyruvate i.e. 2-aminoethanesulfinate Bilophila wadsworthia ?
-
?
hypotaurine + pyruvate purified enzyme Bilophila wadsworthia ?
-
?
hypotaurine + pyruvate i.e. 2-aminoethanesulfinate Bilophila wadsworthia RZATAU ?
-
?
hypotaurine + pyruvate purified enzyme Bilophila wadsworthia RZATAU ?
-
?
additional information no activity with 2-oxoglutarate and phenylpyruvate as amino group acceptor Bilophila wadsworthia ?
-
?
additional information cysteate, glutamate and 2-aminoethanephosphonate are poor amino group donor substrates Bilophila wadsworthia ?
-
?
additional information no activity with 2-oxoglutarate and phenylpyruvate as amino group acceptor Bilophila wadsworthia RZATAU ?
-
?
additional information cysteate, glutamate and 2-aminoethanephosphonate are poor amino group donor substrates Bilophila wadsworthia RZATAU ?
-
?
taurine + 2-oxobutyrate
-
Bilophila wadsworthia 2-sulfoacetaldehyde + 2-aminobutyrate
-
?
taurine + oxaloacetate
-
Bilophila wadsworthia ?
-
?
taurine + pyruvate i.e. 2-aminoethanesulfonate Bilophila wadsworthia 2-sulfoacetaldehyde + L-alanine
-
r
taurine + pyruvate i.e. 2-aminoethanesulfonate Bilophila wadsworthia RZATAU 2-sulfoacetaldehyde + L-alanine
-
r
taurine + pyruvate initial step in anaerobic taurine degradation Bilophila wadsworthia L-alanine + 2-sulfoacetaldehyde
-
?

Subunits

Subunits Comment Organism
More formation of large aggregates depends on storage conditions, freezing at -20°C, or rapid freezing in liquid nitrogen with storage at -20°C increases the 350 kDa form, addition of glycerol decreases the aggregation, a dimer of 95 kDa is preferably formed during storage at 4°C Bilophila wadsworthia
tetramer 4 * 51000, SDS-PAGE Bilophila wadsworthia

Synonyms

Synonyms Comment Organism
tPA
-
Bilophila wadsworthia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Bilophila wadsworthia

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 broad optimum Bilophila wadsworthia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Bilophila wadsworthia

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
enzyme is unstable in buffers with neutral or alkaline pH value, except for HEPES at pH 7.0 Bilophila wadsworthia

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate activating Bilophila wadsworthia
pyridoxal 5'-phosphate pyridoxyl 5'-phosphate is not essentially required Bilophila wadsworthia