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Literature summary for 2.6.1.73 extracted from

  • Chapple, C.C.S.; Glover, J.R.; Ellis, B.E.
    Purification and characterization of methionine:glyoxylate aminotransferase from Brassica carinata and Brassica napus (1990), Plant Physiol., 94, 1887-1896.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.73

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 x * 49000, SDS-PAGE Brassica carinata
51000
-
 x * 51000, SDS-PAGE Brassica napus
230000 290000 gel filtration Brassica napus
230000 290000 gel filtration Brassica carinata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Brassica napus function in the deamination of methionine or production of methionine in the methionine-cycle ?
-
 ?
additional information Brassica carinata function in the deamination of methionine or production of methionine in the methionine-cycle ?
-
 ?
additional information Brassica carinata involved in the biosynthesis of methionine-derived glucosinolates ?
-
 ?

Organism

Organism UniProt Comment Textmining
Brassica carinata
-
 var. R-4218
-
Brassica napus
-
 var. Topas
-

Purification (Commentary)

Purification (Comment) Organism
purification on Mono Q results in isolation of three isoenzymes, MGAT 1, MGAT 2 and MGAT 3 Brassica carinata
var. R-4218, partial Brassica carinata
var. Topas, partial Brassica napus

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Brassica napus
-
leaf
-
 Brassica carinata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
 isoenzyme MGAT 3, 30°C, pH 8.2 Brassica carinata
0.066
-
 MGAT, single enzyme 30°C, pH 8.2 Brassica napus
0.18
-
 isoenzyme MGAT 2 30°C, pH 8.2 Brassica carinata
0.6
-
 isoenzyme MGAT 1, overall activity without separation of isoenzymes 30°C, pH 8.2 Brassica carinata

Storage Stability

Storage Stability Organism
-60°C, desalted enzyme preparation Brassica napus
-60°C, desalted enzyme preparation Brassica carinata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
-
 Brassica napus 2-oxoglutarate + L-methionine
-
 ?
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
-
 Brassica carinata 2-oxoglutarate + L-methionine
-
 ?
L-glutamine + glyoxylate
-
 Brassica napus 2-oxoglutarate + glycine
-
 ?
L-glutamine + glyoxylate
-
 Brassica carinata 2-oxoglutarate + glycine
-
 ?
L-methionine + 4-methylsulfanyl-2-oxobutanoate
-
 Brassica napus 4-methylsulfanyl-2-oxobutanoate + L-methionine
-
 ?
L-methionine + 4-methylsulfanyl-2-oxobutanoate
-
 Brassica carinata 4-methylsulfanyl-2-oxobutanoate + L-methionine
-
 ?
L-methionine + glyoxylate
-
 Brassica napus 4-methylsulfanyl-2-oxobutanoate + glycine
-
 ?
L-methionine + glyoxylate
-
 Brassica carinata 4-methylsulfanyl-2-oxobutanoate + glycine
-
 ?
L-methionine + oxaloacetate 24% of activity compared to glyoxylate Brassica carinata 4-methylsulfanyl-2-oxobutanoate + L-aspartate
-
 ?
additional information function in the deamination of methionine or production of methionine in the methionine-cycle Brassica napus ?
-
 ?
additional information function in the deamination of methionine or production of methionine in the methionine-cycle Brassica carinata ?
-
 ?
additional information involved in the biosynthesis of methionine-derived glucosinolates Brassica carinata ?
-
 ?

Subunits

Subunits Comment Organism
? x * 51000, SDS-PAGE Brassica napus
? x * 49000, SDS-PAGE Brassica carinata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Brassica napus
30
-
 assay at Brassica carinata