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Literature summary for 2.6.1.64 extracted from

  • Hosono, A.; Mizuguchi, H.; Hayashi, H.; Goto, M.; Miyahara, I.; Hirotsu, K.; Kagamiyama, H.
    Glutamine:phenylpyruvate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus HB8 (2003), J. Biochem., 134, 843-851.
    View publication on PubMed
Search for more literature for 2.6.1.64

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 pyruvate pH 8.0, 25°C Thermus thermophilus
0.13
-
 phenylpyruvate pH 8.0, 25°C Thermus thermophilus
0.14
-
 4-hydroxyphenylpyruvate pH 8.0, 25°C Thermus thermophilus
0.16
-
 Indolepyruvate pH 8.0, 25°C Thermus thermophilus
0.76
-
 L-phenylalanine pH 8.0, 25°C Thermus thermophilus
0.82
-
 L-tyrosine pH 8.0, 25°C Thermus thermophilus
1
-
 L-asparagine pH 8.0, 25°C Thermus thermophilus
3.2
-
 L-methionine pH 8.0, 25°C Thermus thermophilus
3.2
-
 L-kynurenine pH 8.0, 25°C Thermus thermophilus
3.5
-
 L-glutamine pH 8.0, 25°C Thermus thermophilus
6
-
 L-tryptophan pH 8.0, 25°C Thermus thermophilus
18
-
 L-histidine pH 8.0, 25°C Thermus thermophilus
19
-
 L-aspartate pH 8.0, 25°C Thermus thermophilus
21
-
 L-glutamate pH 8.0, 25°C Thermus thermophilus
25
-
 2-oxoglutarate pH 8.0, 25°C Thermus thermophilus
37
-
 L-serine pH 8.0, 25°C Thermus thermophilus
39
-
 2-oxoglutarate pH 8.0, 25°C Thermus thermophilus
55
-
 L-threonine pH 8.0, 25°C Thermus thermophilus
63
-
 L-alanine pH 8.0, 25°C Thermus thermophilus
130
-
 L-arginine pH 8.0, 25°C Thermus thermophilus
180
-
 L-leucine pH 8.0, 25°C Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 2 * 42271, calculated, 2 * 42000, SDS-PAGE, N-terminal amino acid sequence Thermus thermophilus
42271
-
 2 * 42271, calculated, 2 * 42000, SDS-PAGE, N-terminal amino acid sequence Thermus thermophilus
82000
-
 gel filtration Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
 expression in Escherichia coli
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 expression in Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + pyruvate
-
 Thermus thermophilus 5-guanidino-2-oxopentanoate + L-alanine
-
 r
L-asparagine + phenylpyruvate
-
 Thermus thermophilus 2-oxosuccinamate + L-phenylalanine
-
 r
L-aspartate + pyruvate
-
 Thermus thermophilus oxaloacetate + L-alanine
-
 r
L-aspartate + pyruvate
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 oxaloacetate + L-alanine
-
 r
L-glutamate + phenylpyruvate
-
 Thermus thermophilus 2-oxoglutarate + L-phenylalanine
-
 r
L-glutamine + pyruvate
-
 Thermus thermophilus 2-oxoglutaramate + L-alanine
-
 ?
L-histidine + pyruvate
-
 Thermus thermophilus 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
 ?
L-histidine + pyruvate
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
 ?
L-kynurenine + pyruvate
-
 Thermus thermophilus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine
-
 r
L-kynurenine + pyruvate
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine
-
 r
L-leucine + phenylpyruvate
-
 Thermus thermophilus 4-methyl-2-oxopentanoate + L-phenylalanine
-
 r
L-methionine + phenylpyruvate
-
 Thermus thermophilus 4-methylsulfanyl-2-oxobutanoate + L-phenylalanine
-
 r
L-methionine + phenylpyruvate
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 4-methylsulfanyl-2-oxobutanoate + L-phenylalanine
-
 r
L-phenylalanine + 2-oxoglutarate
-
 Thermus thermophilus ?
-
 ?
L-phenylalanine + 4-hydroxyphenylpyruvate
-
 Thermus thermophilus phenylpyruvate + L-tyrosine
-
 r
L-phenylalanine + 4-methylsulfanyl-2-oxobutanoate
-
 Thermus thermophilus phenylpyruvate + L-methionine
-
 r
L-phenylalanine + indolepyruvate
-
 Thermus thermophilus phenylpyruvate + L-tryptophan
-
 r
L-phenylalanine + pyruvate
-
 Thermus thermophilus phenylpyruvate + L-alanine
-
 r
L-phenylalanine + pyruvate
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 phenylpyruvate + L-alanine
-
 r
L-serine + pyruvate
-
 Thermus thermophilus 3-hydroxy-2-oxopropanoate + L-alanine
-
 r
L-threonine + pyruvate
-
 Thermus thermophilus 3-hydroxy-2-oxobutanoate + L-alanine
-
 r
L-tryptophan + pyruvate
-
 Thermus thermophilus indolepyruvate + L-alanine
-
 r
L-tyrosine + 2-oxoglutaramate
-
 Thermus thermophilus 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamine
-
 r
phenylpyruvate + L-alanine
-
 Thermus thermophilus L-phenylalanine + pyruvate
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 42271, calculated, 2 * 42000, SDS-PAGE, N-terminal amino acid sequence Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
 L-threonine pH 8.0, 25°C Thermus thermophilus
0.016
-
 L-aspartate pH 8.0, 25°C Thermus thermophilus
0.03
-
 L-glutamate pH 8.0, 25°C Thermus thermophilus
0.12
-
 L-arginine pH 8.0, 25°C Thermus thermophilus
0.13
-
 L-asparagine pH 8.0, 25°C Thermus thermophilus
0.18
-
 L-alanine pH 8.0, 25°C Thermus thermophilus
1.2
-
 L-serine pH 8.0, 25°C Thermus thermophilus
1.3
-
 L-histidine pH 8.0, 25°C Thermus thermophilus
1.5
-
 L-leucine pH 8.0, 25°C Thermus thermophilus
3.1
-
 2-oxoglutarate pH 8.0, 25°C Thermus thermophilus
15
-
 pyruvate pH 8.0, 25°C Thermus thermophilus
130
-
 phenylpyruvate pH 8.0, 25°C Thermus thermophilus
480
-
 Indolepyruvate pH 8.0, 25°C Thermus thermophilus
590
-
 L-phenylalanine pH 8.0, 25°C Thermus thermophilus
620
-
 4-hydroxyphenylpyruvate pH 8.0, 25°C Thermus thermophilus
620
-
 4-methylsulfanyl-2-oxobutanoate pH 8.0, 25°C Thermus thermophilus
830
-
 L-glutamine pH 8.0, 25°C Thermus thermophilus
970
-
 L-tyrosine pH 8.0, 25°C Thermus thermophilus
990
-
 L-tryptophan pH 8.0, 25°C Thermus thermophilus
1100
-
 L-kynurenine pH 8.0, 25°C Thermus thermophilus
1200
-
 L-methionine pH 8.0, 25°C Thermus thermophilus