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Literature summary for 2.6.1.62 extracted from
- 7,8-Diaminopelargonic acid aminotransferase (1979), Methods Enzymol., 62, 342-347.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 7-amino-8-oxononanoate | - |
Escherichia coli |  |
| 7-Keto-8-aminopelargonic acid | competitive with adenosyl-L-methionine | Escherichia coli | |
| adenosine | - |
Escherichia coli | |
| S-Adenosyl-L-(2-hydroxy-4-methylthio)butyric acid | - |
Escherichia coli | |
| S-adenosyl-L-ethionine | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
S-adenosyl-L-methionine | pH 8.5, 37°C | Escherichia coli | |
| 0.001 | - |
7-amino-8-oxononanoate | pH 8.5, 37°C | Escherichia coli | |
| 0.0012 | - |
8-Amino-7-oxononanoate | pH 8.5, 37°C | Escherichia coli | |
| 0.021 | - |
pyridoxamine 5'-phosphate | pH 8.5, 37°C | Escherichia coli | |
| 0.032 | - |
pyridoxal 5'-phosphate | pH 8.5, 37°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
2 * 47000, SDS-PAGE | Escherichia coli |
| 94000 | - |
gel filtration, sucrose density gradient sedimentation | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
regulatory mutant | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 98 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20% glycerol, stable for 1 year | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + 7,8-diketopelargonic acid | - |
Escherichia coli | S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate | - |
? | |
| S-adenosyl-L-methionine + 7-amino-8-oxononanoate | - |
Escherichia coli | S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate | S-adenosyl-4-methylthio-2-oxobutanoate may be decomposed nonenzymatically to form 5'-methylthioadenosine and 2-oxo-3-butenoic acid | ? | |
| S-adenosyl-L-methionine + 8-amino-7-oxononanoate | synonym: 7-keto-8-aminopelargonic acid, KAPA | Escherichia coli | S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate | S-adenosyl-4-methylthio-2-oxobutanoate may be decomposed nonenzymatically to form 5'-methylthioadenosine and 2-oxo-3-butenoic acid | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 47000, SDS-PAGE | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.283 | - |
8-Amino-7-oxononanoate | pH 8.5, 37°C | Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
7-Keto-8-aminopelargonic acid | pH 8.5, 37°C | Escherichia coli | |
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