Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adipic acid | - |
Glycine max | |
Fumaric acid | - |
Glycine max | |
Glutaric acid | - |
Glycine max | |
hydroxylamine | inhibits the aldehyde form of the enzyme while the amino form is found to be inert | Glycine max | |
Maleate | - |
Glycine max | |
Maleic acid | - |
Glycine max | |
phenylhydrazine | - |
Glycine max | |
potassium cyanide | - |
Glycine max | |
Sodium bisulfite | - |
Glycine max | |
succinic acid | - |
Glycine max | |
Thiosemicarbazide | - |
Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.67 | - |
L-leucine | isozyme LAT II, pH 9, 37°C | Glycine max | |
2.3 | - |
2-oxoglutarate | isozyme LAT II, pH 9, 37°C | Glycine max | |
2.5 | - |
L-leucine | isozyme LAT I, pH 9, 37°C | Glycine max | |
3.3 | - |
2-oxoglutarate | isozyme LAT I, pH 9, 37°C | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
gel filtration, isoenzyme LAT I | Glycine max |
93300 | - |
gel filtration, isoenzyme LAT II | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine + 2-oxoglutarate | Glycine max | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
soybean, var. Kali tur | - |
Purification (Comment) | Organism |
---|---|
partially, 2 isoenzymes, LAT I and LAT II | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.4 | - |
isoenzyme LAT I | Glycine max |
8.7 | - |
isoenzyme LAT II | Glycine max |
Storage Stability | Organism |
---|---|
4°C, concentrated enzyme, activity is stable for 2 months | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine + 2-oxoglutarate | - |
Glycine max | 4-methyl-2-oxopentanoate + L-glutamate | - |
? | |
L-leucine + 2-oxoglutarate | - |
Glycine max | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 4-methyl-2-oxopentanoate | - |
Glycine max | 4-methyl-2-oxopentanoate + L-leucine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.9 | - |
- |
Glycine max |