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Literature summary for 2.6.1.6 extracted from

  • Pathre, U.; Singh, A.K.; Viswanathan, P.N.; Sane, P.V.
    Purification and properties of leucine aminotransferase from soybean seedlings (1987), Phytochemistry, 26, 2913-2917.
No PubMed abstract available
Search for more literature for 2.6.1.6

Inhibitors

Inhibitors Comment Organism Structure
adipic acid
-
 Glycine max
Fumaric acid
-
 Glycine max
Glutaric acid
-
 Glycine max
hydroxylamine inhibits the aldehyde form of the enzyme while the amino form is found to be inert Glycine max
Maleate
-
 Glycine max
Maleic acid
-
 Glycine max
phenylhydrazine
-
 Glycine max
potassium cyanide
-
 Glycine max
Sodium bisulfite
-
 Glycine max
succinic acid
-
 Glycine max
Thiosemicarbazide
-
 Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.67
-
 L-leucine isozyme LAT II, pH 9, 37°C Glycine max
2.3
-
 2-oxoglutarate isozyme LAT II, pH 9, 37°C Glycine max
2.5
-
 L-leucine isozyme LAT I, pH 9, 37°C Glycine max
3.3
-
 2-oxoglutarate isozyme LAT I, pH 9, 37°C Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 gel filtration, isoenzyme LAT I Glycine max
93300
-
 gel filtration, isoenzyme LAT II Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-leucine + 2-oxoglutarate Glycine max
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Glycine max
-
 soybean, var. Kali tur
-

Purification (Commentary)

Purification (Comment) Organism
partially, 2 isoenzymes, LAT I and LAT II Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.4
-
 isoenzyme LAT I Glycine max
8.7
-
 isoenzyme LAT II Glycine max

Storage Stability

Storage Stability Organism
4°C, concentrated enzyme, activity is stable for 2 months Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-leucine + 2-oxoglutarate
-
 Glycine max 4-methyl-2-oxopentanoate + L-glutamate
-
 ?
L-leucine + 2-oxoglutarate
-
 Glycine max 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-leucine + 4-methyl-2-oxopentanoate
-
 Glycine max 4-methyl-2-oxopentanoate + L-leucine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.9
-
-
 Glycine max