Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | slight inhibition | Rattus norvegicus | |
p-chloromercuribenzoate | 1.0 mM, complete inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
2-oxoglutarate | pH 8.2, 37°C | Rattus norvegicus | |
25 | - |
leucine | pH 8.2, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | predominantly found in mitochondrion | Rattus norvegicus | 5737 | - |
microsome | - |
Rattus norvegicus | - |
- |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
nucleus | - |
Rattus norvegicus | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine + 2-oxoglutarate | Rattus norvegicus | leucine metabolism and hence of cholesterogenesis and ketogenesis, not present in fetal rat liver, but after birth activity appears and increases rapidly | 4-methyl-2-oxopentanoate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.033 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine + 2-oxoglutarate | - |
Rattus norvegicus | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | leucine metabolism and hence of cholesterogenesis and ketogenesis, not present in fetal rat liver, but after birth activity appears and increases rapidly | Rattus norvegicus | 4-methyl-2-oxopentanoate + L-glutamate | - |
? | |
L-leucine + oxaloacetate | - |
Rattus norvegicus | 4-methyl-2-oxopentanoate + L-aspartate | - |
? | |
additional information | specific for leucine, isoleucine or valine, no other amino acid would serve as an amino donor, pyruvate is no amino acid acceptor | Rattus norvegicus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
- |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | Km 0.004 mM | Rattus norvegicus |