Cloned (Comment) | Organism |
---|---|
gene StsC identified, cloned by PCR and expressed in Escherichia coli BL21 and Streptomyces lividans | Streptomyces griseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Streptomyces griseus |
45500 | - |
SDS-PAGE | Streptomyces griseus |
95000 | 100000 | gel filtration | Streptomyces griseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone | Streptomyces bikiniensis | - |
2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol | - |
r | |
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone | Streptomyces hygroscopicus | - |
2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol | - |
r | |
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone | Streptomyces griseus | streptidine subpathway of streptomycin biosynthesis | 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol | - |
r | |
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone | Streptomyces bikiniensis NRRL 1049b | - |
2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Escherichia coli | - |
host strain, free of enzymatic activity | - |
no activity in Streptomyces lividans | - |
66 strain TK23, host strains, free of enzymatic activity | - |
no activity in Streptomyces lividans | - |
66 strain 1326 | - |
Streptomyces bikiniensis | - |
NRRL 1049b | - |
Streptomyces bikiniensis NRRL 1049b | - |
NRRL 1049b | - |
Streptomyces griseus | - |
N2-3-11, DSM 40236 | - |
Streptomyces hygroscopicus | - |
subsp. glebosus DSM 40823 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, pJAW76-2, expressed in Streptomyces lividans | Streptomyces griseus |
Storage Stability | Organism |
---|---|
6°C, purified enzyme stable for several weeks | Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone | - |
Streptomyces griseus | 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol | - |
r | |
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone | - |
Streptomyces bikiniensis | 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol | - |
r | |
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone | - |
Streptomyces hygroscopicus | 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol | - |
r | |
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone | streptidine subpathway of streptomycin biosynthesis | Streptomyces griseus | 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol | - |
r | |
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone | - |
Streptomyces bikiniensis NRRL 1049b | 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol | - |
r | |
L-glutamine + scyllo-inosose | - |
Streptomyces griseus | scyllo-inosamine + 2-oxoglutaramate | spontaneously cyclization of 2-oxoglutaramate to 2-pyrrolidone-5-hydroxy-5-carboxylic acid | r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, SDS-PAGE | Streptomyces griseus |