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Literature summary for 2.6.1.5 extracted from
- Tyrosine aminotransferase contributes to benzylisoquinoline alkaloid biosynthesis in opium poppy (2011), Plant Physiol., 157, 1067-1078.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
2-oxoglutarate | pH 8.5, 30°C | Papaver somniferum |  |
| 1.82 | - |
L-tyrosine | pH 8.5, 30°C | Papaver somniferum | |
| 2.45 | - |
pyruvate | pH 8.5, 30°C | Papaver somniferum | |
| 6.33 | - |
L-phenylalanine | pH 8.5, 30°C | Papaver somniferum | |
| 7.83 | - |
L-tryptophan | pH 8.5, 30°C | Papaver somniferum | |
| 56.13 | - |
oxaloacetate | pH 8.5, 30°C | Papaver somniferum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
- |
Papaver somniferum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Papaver somniferum | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | transcripts highly detected | Papaver somniferum | - |
| stem | transcripts highly detected | Papaver somniferum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-phenylalanine + 2-oxoglutarate | - |
Papaver somniferum | 2-oxo-3-phenylpropanoate + L-glutamate | - |
? | |
| L-tryptophan + 2-oxoglutarate | - |
Papaver somniferum | 3-(1H-indol-3-yl)-2-oxopropanoate + L-glutamate | - |
? | |
| L-tryptophan + oxaloacetate | - |
Papaver somniferum | 3-(1H-indol-3-yl)-2-oxopropanoate + L-glycine | - |
? | |
| L-tryptophan + pyruvate | - |
Papaver somniferum | 3-(1H-indol-3-yl)-2-oxopropanoate + L-alanine | - |
? | |
| L-tyrosine + 2-oxoglutarate | - |
Papaver somniferum | 4-hydroxyphenylpyruvate + L-glutamate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TyrAT | - |
Papaver somniferum |
| tyrosine aminotransferase | - |
Papaver somniferum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Papaver somniferum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
L-phenylalanine | pH 8.5, 30°C | Papaver somniferum | |
| 0.22 | - |
2-oxoglutarate | pH 8.5, 30°C | Papaver somniferum | |
| 0.24 | - |
L-tyrosine | pH 8.5, 30°C | Papaver somniferum | |
| 0.34 | - |
pyruvate | pH 8.5, 30°C | Papaver somniferum | |
| 0.36 | - |
L-tryptophan | pH 8.5, 30°C | Papaver somniferum | |
| 0.37 | - |
oxaloacetate | pH 8.5, 30°C | Papaver somniferum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Papaver somniferum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | virus-induced gene silencing is used to evaluate the contribution of TyrAT to benzylisoquinoline alkaloids metabolism in opium poppy. TyrAT transcript levels were reduced by at least 80% in silenced plants compared with controls and show a moderate reduction in total alkaloid content | Papaver somniferum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
oxaloacetate | pH 8.5, 30°C | Papaver somniferum | |
| 0.01 | - |
L-phenylalanine | pH 8.5, 30°C | Papaver somniferum | |
| 0.13 | - |
L-tyrosine | pH 8.5, 30°C | Papaver somniferum | |
| 0.14 | - |
pyruvate | pH 8.5, 30°C | Papaver somniferum | |
| 0.63 | - |
2-oxoglutarate | pH 8.5, 30°C | Papaver somniferum | |
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Release 2023.1 (January 2023)
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