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Literature summary for 2.6.1.5 extracted from

  • Prabhu, P.R.; Hudson, A.O.
    Identification and partial characterization of an L-tyrosine aminotransferase (TAT) from Arabidopsis thaliana (2010), Biochem. Res. Int., 2010, 549572.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 2-oxo-3-phenylpropanoate pH 8.2, 30°C Arabidopsis thaliana
0.19
-
 2-oxoglutarate pH 8.2, 30°C Arabidopsis thaliana
0.19
-
 L-tyrosine pH 8.2, 30°C Arabidopsis thaliana
0.22
-
 3-(4-hydroxyphenyl)-2-oxopropanoate pH 8.2, 30°C Arabidopsis thaliana
0.84
-
 L-phenylalanine pH 8.2, 30°C Arabidopsis thaliana
1.4
-
 L-glutamate pH 8.2, 30°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LVY1
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 Arabidopsis thaliana L-tyrosine + 2-oxoglutarate
-
 r
L-phenylalanine + 2-oxoglutarate
-
 Arabidopsis thaliana 2-oxo-3-phenylpropanoate + L-glutamate
-
 r
L-tyrosine + 2-oxoglutarate best substrate Arabidopsis thaliana 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 r
L-tyrosine + 2-oxopentanoate relative activity compared to 2-oxoglutarate: 3% Arabidopsis thaliana 3-(4-hydroxyphenyl)-2-oxopropanoate + (2S)-2-aminopentanoate
-
 ?
L-tyrosine + oxaloacetate relative activity compared to 2-oxoglutarate: 75% Arabidopsis thaliana ?
-
 ?
L-tyrosine + prephenate relative activity compared to 2-oxoglutarate: 23% Arabidopsis thaliana ?
-
 ?
L-tyrosine + pyruvate relative activity compared to 2-oxoglutarate: 40% Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
At5g36160
-
 Arabidopsis thaliana
TAT
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 assay at Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism in vivo analysis by functional complementation shows that the gene is able to complement an Escherichia coli with a background of aminotransferase mutations that confers auxotrophy for L-tyrosine and L-phenylalanine Arabidopsis thaliana