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Literature summary for 2.6.1.46 extracted from

  • Vandenende, C.S.; Vlasschaert, M.; Seah, S.Y.K.
    Functional characterization of an aminotransferase required for pyoverdine siderophore biosynthesis in Pseudomonas aeruginosa PAO1 (2004), J. Bacteriol., 186, 5596-5602.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.46

Cloned(Commentary)

Cloned (Comment) Organism
gene pvdH, DNA sequence determination and analysis, subcloning and expression in Escherichia coli, overexpression in enzyme-knockout Pseudomonas aeruginosa strain PAO1 Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetic analysis, enzyme shows a ping-pong kinetic mechanism Pseudomonas aeruginosa
0.18
-
 2-oxoglutarate pH 7.5, 25°C, recombinant enzyme Pseudomonas aeruginosa
0.26
-
 L-2,4-diaminobutanoate pH 7.5, 25°C, with 2-oxoglutarate, recombinant enzyme Pseudomonas aeruginosa
0.29
-
 L-2,4-diaminobutanoate pH 7.5, 25°C, with pyruvate, recombinant enzyme Pseudomonas aeruginosa
6.2
-
 pyruvate pH 7.5, 25°C, recombinant enzyme Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information iron-limiting conditions induce pyoverdine siderophore biosynthesis including enzyme activity Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 4 * 50000, recombinant enzyme, SDS-PAGE Pseudomonas aeruginosa
183000
-
 recombinant enzyme, gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa enzyme is involved in biosynthesis of the pyoverdine siderophore ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 gene pvdH
-

Purification (Commentary)

Purification (Comment) Organism
221fold, recombinantly overexpressed enzyme from Pseudomonas aeruginosa strain PAO1 by anion exchange chromatography and gel filtration to 90% purity Pseudomonas aeruginosa

Reaction

Reaction Comment Organism Reaction ID
L-2,4-diaminobutanoate + pyruvate = L-aspartate 4-semialdehyde + L-alanine ping-pong kinetic mechanism Pseudomonas aeruginosa
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.21
-
 purified recombinant enzyme, coupled assay with homoserine dehydrogenase from Saccharomyces cerevisiae Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2,4-diaminobutanoate + 2-oxoglutarate 2-oxoglutarate is the best amino donor substrate Pseudomonas aeruginosa L-aspartate 4-semialdehyde + L-glutamate
-
 r
L-2,4-diaminobutanoate + pyruvate specificty for pyruvate is 41fold lower compared to 2-oxoglutarate Pseudomonas aeruginosa L-aspartate 4-semialdehyde + L-alanine
-
 r
additional information enzyme is involved in biosynthesis of the pyoverdine siderophore Pseudomonas aeruginosa ?
-
 ?
additional information no activity with 2-oxobutyrate as amino acceptor Pseudomonas aeruginosa ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, recombinant enzyme, SDS-PAGE Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.39
-
 pyruvate pH 7.5, 25°C, recombinant enzyme Pseudomonas aeruginosa
1.39
-
 2-oxoglutarate pH 7.5, 25°C, recombinant enzyme Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Pseudomonas aeruginosa