Cloned (Comment) | Organism |
---|---|
gene pvdH, DNA sequence determination and analysis, subcloning and expression in Escherichia coli, overexpression in enzyme-knockout Pseudomonas aeruginosa strain PAO1 | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic analysis, enzyme shows a ping-pong kinetic mechanism | Pseudomonas aeruginosa | |
0.18 | - |
2-oxoglutarate | pH 7.5, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
0.26 | - |
L-2,4-diaminobutanoate | pH 7.5, 25°C, with 2-oxoglutarate, recombinant enzyme | Pseudomonas aeruginosa | |
0.29 | - |
L-2,4-diaminobutanoate | pH 7.5, 25°C, with pyruvate, recombinant enzyme | Pseudomonas aeruginosa | |
6.2 | - |
pyruvate | pH 7.5, 25°C, recombinant enzyme | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | iron-limiting conditions induce pyoverdine siderophore biosynthesis including enzyme activity | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
4 * 50000, recombinant enzyme, SDS-PAGE | Pseudomonas aeruginosa |
183000 | - |
recombinant enzyme, gel filtration | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | enzyme is involved in biosynthesis of the pyoverdine siderophore | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
gene pvdH | - |
Purification (Comment) | Organism |
---|---|
221fold, recombinantly overexpressed enzyme from Pseudomonas aeruginosa strain PAO1 by anion exchange chromatography and gel filtration to 90% purity | Pseudomonas aeruginosa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-2,4-diaminobutanoate + pyruvate = L-aspartate 4-semialdehyde + L-alanine | ping-pong kinetic mechanism | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.21 | - |
purified recombinant enzyme, coupled assay with homoserine dehydrogenase from Saccharomyces cerevisiae | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2,4-diaminobutanoate + 2-oxoglutarate | 2-oxoglutarate is the best amino donor substrate | Pseudomonas aeruginosa | L-aspartate 4-semialdehyde + L-glutamate | - |
r | |
L-2,4-diaminobutanoate + pyruvate | specificty for pyruvate is 41fold lower compared to 2-oxoglutarate | Pseudomonas aeruginosa | L-aspartate 4-semialdehyde + L-alanine | - |
r | |
additional information | enzyme is involved in biosynthesis of the pyoverdine siderophore | Pseudomonas aeruginosa | ? | - |
? | |
additional information | no activity with 2-oxobutyrate as amino acceptor | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 50000, recombinant enzyme, SDS-PAGE | Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.39 | - |
pyruvate | pH 7.5, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.39 | - |
2-oxoglutarate | pH 7.5, 25°C, recombinant enzyme | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas aeruginosa |