Literature summary for 2.6.1.44 extracted from

Noguchi, T.; Okuno, E.; Takada, Y.; Minatogawa, Y.; Okai, K.; Kido, R.
Characteristics of hepatic alanine-glyoxylate aminotransferase in different mammalian species (1978), Biochem. J., 169, 113-122.

Activating Compound

Activating Compound	Comment	Organism
glucagon	induced by, isoenzyme 1 is increased in activity	Mus musculus
glucagon	induced by, isoenzyme 1 is increased in activity	Rattus norvegicus
glucagon	induced by, isoenzyme 1 is increased in activity	Canis lupus familiaris
glucagon	induced by, isoenzyme 1 is increased in activity	Felis catus

Inhibitors

Inhibitors	Comment	Organism
hydroxylamine	isoenzyme 1	Canis lupus familiaris
hydroxylamine	isoenzyme 1	Felis catus
hydroxylamine	isoenzyme; isoenzyme 1	Mus musculus
hydroxylamine	isoenzyme; isoenzyme 1	Rattus norvegicus
Isonicotinic acid hydrazide	isoenzyme 1 and isoenzyme 2	Mus musculus
Isonicotinic acid hydrazide	isoenzyme 1 and isoenzyme 2	Rattus norvegicus
Semicarbazide	isoenzyme 1	Canis lupus familiaris
Semicarbazide	isoenzyme 1	Felis catus
Semicarbazide	isoenzyme; isoenzyme 1	Mus musculus
Semicarbazide	isoenzyme; isoenzyme 1	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.07	-	glyoxylate	pH 8.2, 37°C, isoenzyme 1, alanine as amino donor	Rattus norvegicus
0.1	-	glyoxylate	pH 8.2, 37°C, isoenzyme 1, alanine as amino donor	Felis catus
0.13	-	glyoxylate	pH 8.2, 37°C, isoenzyme 1, alanine as amino donor	Canis lupus familiaris
0.2	-	glyoxylate	pH 8.2, 37°C, isoenzyme 1, alanine as amino donor	Mus musculus
0.72	-	glyoxylate	pH 8.0, 37°C, alanine as amino donor	Mus musculus
1	-	glyoxylate	pH 8.2, 37°C, isoenzyme 2, alanine as amino donor	Rattus norvegicus
1.4	-	L-alanine	pH 8.2, 37°C, isoenzyme 1, glyoxylate as amino acceptor	Mus musculus
2.1	-	L-alanine	pH 8.2, 37°C, isoenzyme 1, glyoxylate as amino acceptor	Rattus norvegicus
3	-	L-alanine	pH 8.2, 37°C, isoenzyme 1, glyoxylate as amino acceptor	Canis lupus familiaris
3.9	-	L-alanine	pH 8.2, 37°C, isoenzyme 1, glyoxylate as amino acceptor	Canis lupus familiaris
25	-	L-alanine	pH 8.2, 37°C, isoenzyme 2, glyoxylate as amino acceptor	Mus musculus
30	-	L-alanine	pH 8.2, 37°C, isoenzyme 2, glyoxylate as amino acceptor	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Rattus norvegicus	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	2 * 38000, isoenzyme 1, SDS-PAGE	Mus musculus
38000	-	2 * 38000, isoenzyme 1, SDS-PAGE	Rattus norvegicus
38000	-	2 * 38000, isoenzyme 1, SDS-PAGE	Canis lupus familiaris
38000	-	2 * 38000, isoenzyme 1, SDS-PAGE	Felis catus
77000	-	-	Mus musculus
77000	-	-	Canis lupus familiaris
77000	-	-	Felis catus
77000	-	isoenzyme 1, gel filtration	Rattus norvegicus
80000	-	sucrose-density-gradient centrifugation	Mus musculus
80000	-	sucrose-density-gradient centrifugation	Rattus norvegicus
80000	-	sucrose-density-gradient centrifugation	Canis lupus familiaris
80000	-	sucrose-density-gradient centrifugation	Felis catus
80000	-	peroxisomal and mitochondrial alanine-glyoxylate aminotransferase 1	Rattus norvegicus
170000	-	isoenzyme 2, gel filtration	Rattus norvegicus
175000	-	-	Canis lupus familiaris
175000	-	liver isoenzyme 2, gel filtration	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
L-alanine + glyoxylate	Mus musculus	-	pyruvate + glycine	-	ir
L-alanine + glyoxylate	Rattus norvegicus	-	pyruvate + glycine	-	ir
L-alanine + glyoxylate	Canis lupus familiaris	-	pyruvate + glycine	-	ir
L-alanine + glyoxylate	Felis catus	-	pyruvate + glycine	-	ir

Organism

Organism	UniProt	Comment	Textmining
Canis lupus familiaris	-	dog	-
Felis catus	-	cat	-
Mus musculus	-	mouse	-
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
isoenzyme 1	Canis lupus familiaris
isoenzyme 1	Felis catus
isoenzyme 1 and 2	Mus musculus
isoenzyme 1 and 2	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Mus musculus	-
liver	-	Rattus norvegicus	-
liver	-	Canis lupus familiaris	-
liver	-	Felis catus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.64	-	isoenzyme 2	Canis lupus familiaris
2.93	-	-	Rattus norvegicus
2.93	-	isoenzyme 2	Canis lupus familiaris
21.4	-	isoenzyme 1	Rattus norvegicus
23.2	-	isoenzyme 1	Mus musculus
86.3	-	isoenzyme 1	Felis catus
91	-	isoenzyme 1	Canis lupus familiaris
160	-	isoenzyme 1	Rattus norvegicus

Storage Stability

Storage Stability	Organism
-20°C, 50 mM potassium phosphate buffer, 0.1 mM pyridoxal 5'-phosphate, 1 mM 2-mercaptoethanol, may be stored for at least 4 weeks without loss of either activity	Rattus norvegicus
-20°C, 50 mM potassium phosphate buffer, pH 7.5, 0.1 mM pyridoxal 5'-phosphate, 1 mM 2-mercaptoethanol, may be stored for at least 4 weeks without loss of either activity	Mus musculus
-20°C, 50 mM potassium phosphate buffer, pH 7.5, 0.1 mM pyridoxal 5'-phosphate, 1 mM 2-mercaptoethanol, may be stored for at least 4 weeks without loss of either activity	Canis lupus familiaris
-20°C, 50 mM potassium phosphate buffer, pH 7.5, 0.1 mM pyridoxal 5'-phosphate, 1 mM 2-mercaptoethanol, may be stored for at least 4 weeks without loss of either activity	Felis catus
0-6°C, 50 mM potassium phosphate buffer, pH 7.5, 0.1 mM pyridoxal 5'-phosphate, 1 mM 2-mercaptoethanol, may be stored for at least 2 weeks with little loss of either activity	Mus musculus
0-6°C, 50 mM potassium phosphate buffer, pH 7.5, 0.1 mM pyridoxal 5'-phosphate, 1 mM 2-mercaptoethanol, may be stored for at least 2 weeks with little loss of either activity	Rattus norvegicus
0-6°C, 50 mM potassium phosphate buffer, pH 7.5, 0.1 mM pyridoxal 5'-phosphate, 1 mM 2-mercaptoethanol, may be stored for at least 2 weeks with little loss of either activity	Canis lupus familiaris
0-6°C, 50 mM potassium phosphate buffer, pH 7.5, 0.1 mM pyridoxal 5'-phosphate, 1 mM 2-mercaptoethanol, may be stored for at least 2 weeks with little loss of either activity	Felis catus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
L-alanine + glyoxylate	-	Mus musculus	pyruvate + glycine	-	ir
L-alanine + glyoxylate	-	Rattus norvegicus	pyruvate + glycine	-	ir
L-alanine + glyoxylate	-	Canis lupus familiaris	pyruvate + glycine	-	ir
L-alanine + glyoxylate	-	Felis catus	pyruvate + glycine	-	ir
L-alanine + glyoxylate	isoenzyme 1	Mus musculus	pyruvate + glycine	-	ir
L-alanine + glyoxylate	isoenzyme 1	Rattus norvegicus	pyruvate + glycine	-	ir
L-alanine + glyoxylate	isoenzyme 2 highly specific, little or no activity with other amino donors/acceptors	Mus musculus	pyruvate + glycine	-	ir
L-alanine + glyoxylate	isoenzyme 2 highly specific, little or no activity with other amino donors/acceptors	Rattus norvegicus	pyruvate + glycine	-	ir
L-alanine + hydroxypyruvate	-	Canis lupus familiaris	pyruvate + L-serine	-	ir
L-alanine + hydroxypyruvate	-	Felis catus	pyruvate + L-serine	-	ir
L-alanine + hydroxypyruvate	isoenzyme 1	Mus musculus	pyruvate + L-serine	-	ir
L-alanine + hydroxypyruvate	isoenzyme 1	Rattus norvegicus	pyruvate + L-serine	-	ir
L-alanine + phenylpyruvate	isoenzyme 1	Mus musculus	pyruvate + L-phenylalanine	-	ir
L-alanine + phenylpyruvate	isoenzyme 1	Rattus norvegicus	pyruvate + L-phenylalanine	-	ir
L-alanine + phenylpyruvate	isoenzyme 1, less effectively acceptor	Canis lupus familiaris	pyruvate + L-phenylalanine	-	ir
L-alanine + phenylpyruvate	isoenzyme 1, less effectively acceptor	Felis catus	pyruvate + L-phenylalanine	-	ir
L-asparagine + glyoxylate	isoenzyme 1	Mus musculus	4-amino-2,4-dioxobutanoate + glycine	-	ir
L-asparagine + glyoxylate	isoenzyme 1	Rattus norvegicus	4-amino-2,4-dioxobutanoate + glycine	-	ir
L-glutamine + glyoxylate	isoenzyme 1	Mus musculus	2-oxoglutaramate + glycine	-	ir
L-glutamine + glyoxylate	isoenzyme 1	Rattus norvegicus	2-oxoglutaramate + glycine	-	ir
L-histidine + glyoxylate	isoenzyme 1	Mus musculus	3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine	-	ir
L-histidine + glyoxylate	isoenzyme 1	Rattus norvegicus	3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine	-	ir
L-isoleucine + glyoxylate	isoenzyme 1	Mus musculus	3-methyl-2-oxopropanoate + glycine	-	ir
L-leucine + glyoxylate	isoenzyme 1	Mus musculus	4-methyl-2-oxopentanoate + glycine	-	ir
L-leucine + glyoxylate	isoenzyme 1	Rattus norvegicus	4-methyl-2-oxopentanoate + glycine	-	ir
L-methionine + glyoxylate	isoenzyme 1	Mus musculus	4-methylsulfanyl-2-oxobutanoate + glycine	-	ir
L-methionine + glyoxylate	isoenzyme 1	Rattus norvegicus	4-methylsulfanyl-2-oxobutanoate + glycine	-	ir
L-phenylalanine + glyoxylate	isoenzyme 1	Mus musculus	phenylpyruvate + glycine	-	ir
L-phenylalanine + glyoxylate	isoenzyme 1	Rattus norvegicus	phenylpyruvate + glycine	-	ir
L-serine + glyoxylate	-	Canis lupus familiaris	3-hydroxy-2-oxopropanoate + glycine	-	ir
L-serine + glyoxylate	-	Felis catus	3-hydroxy-2-oxopropanoate + glycine	-	ir
L-serine + glyoxylate	isoenzyme 1	Mus musculus	3-hydroxy-2-oxopropanoate + glycine	-	ir
L-serine + glyoxylate	isoenzyme 1	Rattus norvegicus	3-hydroxy-2-oxopropanoate + glycine	-	ir
L-serine + pyruvate	isoenzyme 1	Mus musculus	3-hydroxy-2-oxopropanoate + L-alanine	-	ir
L-serine + pyruvate	isoenzyme 1	Rattus norvegicus	3-hydroxy-2-oxopropanoate + L-alanine	-	ir
L-serine + pyruvate	isoenzyme 1	Canis lupus familiaris	3-hydroxy-2-oxopropanoate + L-alanine	-	ir
L-serine + pyruvate	isoenzyme 1	Felis catus	3-hydroxy-2-oxopropanoate + L-alanine	-	ir
L-tyrosine + glyoxylate	isoenzyme 1	Mus musculus	3-(4-hydroxyphenyl)-2-oxopropanoate + glycine	-	ir
L-tyrosine + glyoxylate	isoenzyme 1	Rattus norvegicus	3-(4-hydroxyphenyl)-2-oxopropanoate + glycine	-	ir
L-valine + glyoxylate	isoenzyme 1	Mus musculus	3-methyl-2-oxobutanoate + glycine	-	ir
additional information	isoenzyme 1, little or no activity with 2-oxoglutarate as amino acceptor and alanine, serine, glutamic acid, isoleucine, methionine, glutamine, asparagine, valine, aspartic acid, leucine, phenylalanine, tyrosine, histidine, tryptophan or 5-hydroxytryptophan	Mus musculus	?	-	?
additional information	isoenzyme 1, little or no activity with 2-oxoglutarate as amino acceptor and alanine, serine, glutamic acid, isoleucine, methionine, glutamine, asparagine, valine, aspartic acid, leucine, phenylalanine, tyrosine, histidine, tryptophan or 5-hydroxytryptophan	Rattus norvegicus	?	-	?
additional information	isoenzyme 1, little or no activity with 2-oxoglutarate as amino acceptor and alanine, serine, glutamic acid, isoleucine, methionine, glutamine, asparagine, valine, aspartic acid, leucine, phenylalanine, tyrosine, histidine, tryptophan or 5-hydroxytryptophan	Canis lupus familiaris	?	-	?
additional information	isoenzyme 1, little or no activity with 2-oxoglutarate as amino acceptor and alanine, serine, glutamic acid, isoleucine, methionine, glutamine, asparagine, valine, aspartic acid, leucine, phenylalanine, tyrosine, histidine, tryptophan or 5-hydroxytryptophan	Felis catus	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 38000, isoenzyme 1, SDS-PAGE	Mus musculus
dimer	2 * 38000, isoenzyme 1, SDS-PAGE	Rattus norvegicus
dimer	2 * 38000, isoenzyme 1, SDS-PAGE	Canis lupus familiaris
dimer	2 * 38000, isoenzyme 1, SDS-PAGE	Felis catus

Synonyms

Synonyms	Comment	Organism
EC 2.6.1.51	serine-pyruvate aminotransferase identical with liver isoenzyme 1	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.1	8.4	isoenzyme 2	Mus musculus
8.3	8.6	-	Mus musculus
8.3	8.6	-	Rattus norvegicus
8.3	8.6	-	Canis lupus familiaris
8.3	8.6	-	Felis catus
8.5	8.8	isoenzyme 2	Rattus norvegicus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Mus musculus	isoelectric focusing on a pH 3.5-10 ampholine gradient	-	5.9
Rattus norvegicus	isoelectric focusing on a pH 3.5-10 ampholine gradient	-	6