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Literature summary for 2.6.1.43 extracted from

  • Rhee, H.i.; Murata, K.; Kimura, A.
    L-alanine: 4,5-dioxovalerate aminotransferase from Pseudomonas riboflavina: purification and inactivation by methylglyoxal (1988), J. Biochem., 103, 1045-1049.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallized as bright yellow needles Devosia riboflavina

Inhibitors

Inhibitors Comment Organism Structure
hemin
-
Devosia riboflavina
methylglyoxal
-
Devosia riboflavina
pyruvate
-
Devosia riboflavina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.75
-
4,5-Dioxopentanoate pH 8.0, 37°C Devosia riboflavina
1.7
-
L-alanine pH 8.0, 37°C Devosia riboflavina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
4 * 48000, SDS-PAGE Devosia riboflavina
190000
-
gel filtration Devosia riboflavina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + 4,5-dioxopentanoate Devosia riboflavina delta-aminolevulinate pathway 5-aminolevulinate + pyruvate
-
ir

Organism

Organism UniProt Comment Textmining
Devosia riboflavina
-
IFO 3140
-

Purification (Commentary)

Purification (Comment) Organism
-
Devosia riboflavina

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.02
-
-
Devosia riboflavina

Storage Stability

Storage Stability Organism
-15°C, stable for 3 months Devosia riboflavina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-alanine + 4,5-dioxopentanoate 39% of the activity with L-alanine Devosia riboflavina 5-aminolevulinate + malonic semialdehyde
-
ir
epsilon-amino-n-caproate + 4,5-dioxopentanoate 34% of the activity with L-alanine Devosia riboflavina 5-aminolevulinate + 6-oxo-n-hexanoate
-
ir
gamma-amino-n-butyrate + 4,5-dioxopentanoate 55% of the activity with L-alanine Devosia riboflavina 5-aminolevulinate + succinic semialdehyde
-
ir
L-alanine + 4,5-dioxopentanoate
-
Devosia riboflavina 5-aminolevulinate + pyruvate
-
ir
L-alanine + 4,5-dioxopentanoate delta-aminolevulinate pathway Devosia riboflavina 5-aminolevulinate + pyruvate
-
ir
L-ornithine + 4,5-dioxopentanoate 14% of the activity with L-alanine Devosia riboflavina 5-aminolevulinate + 5-amino-2-oxopentanoate
-
ir
additional information L-lysine, L-arginine, L-glutamate, L-methionine, L-histidine, glycine, L-phenylalanine, L-threonine, L-asparagine, L-serine, L-proline, L-valine, L-isoleucine, L-leucine, L-tyrosine, L-cysteine and L-tryptophan are all inert as amino donors, methylglyoxal is a poor substrate for the enzyme Devosia riboflavina ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 48000, SDS-PAGE Devosia riboflavina

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Devosia riboflavina