General Stability | Organism |
---|---|
glutathione or beta-mercaptoethanol restores activity of aged enzyme preparations, optimally at 1.0 mM | Cereibacter sphaeroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4,5-Dioxopentanoate | substrate inhibition | Cereibacter sphaeroides | |
acetyl acetone | - |
Cereibacter sphaeroides | |
amino-oxyacetic acid | - |
Cereibacter sphaeroides | |
benzoyl acetone | - |
Cereibacter sphaeroides | |
beta-Alanine | 8% inhibition | Cereibacter sphaeroides | |
beta-Iodopropionate | 30% inhibition | Cereibacter sphaeroides | |
cadaverine | 14% inhibition | Cereibacter sphaeroides | |
cysteamine | - |
Cereibacter sphaeroides | |
cysteine | 90% inhibition | Cereibacter sphaeroides | |
diacetyl | - |
Cereibacter sphaeroides | |
ethyl acetoacetate | - |
Cereibacter sphaeroides | |
glyoxylic acid | - |
Cereibacter sphaeroides | |
homocysteine | - |
Cereibacter sphaeroides | |
iodoacetate | 30% inhibition | Cereibacter sphaeroides | |
Isonicotinic acid hydrazide | - |
Cereibacter sphaeroides | |
lysine | 20% inhibition | Cereibacter sphaeroides | |
additional information | iodoacetamide and chloroacetamide are not inhibitors | Cereibacter sphaeroides | |
ophthalaldehyde | - |
Cereibacter sphaeroides | |
ornithine | 20% inhibition | Cereibacter sphaeroides | |
oxaloacetic acid | - |
Cereibacter sphaeroides | |
p-chloromercuribenzoate | 78% inhibition | Cereibacter sphaeroides | |
Penicillamine | - |
Cereibacter sphaeroides | |
Pyruvic acid | - |
Cereibacter sphaeroides | |
succinic acid | - |
Cereibacter sphaeroides | |
Thioglycollate | 74% inhibition | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
4,5-Dioxopentanoate | pH 7.0, 37°C | Cereibacter sphaeroides | |
8 | - |
L-alanine | pH 7.0, 37°C | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + 4,5-dioxopentanoate | Cereibacter sphaeroides | - |
5-aminolevulinate + pyruvate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Cereibacter sphaeroides |
Storage Stability | Organism |
---|---|
-15°C, stable for several months | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
delta-amino-n-valerate + 4,5-dioxopentanoate | transaminate at 95% of the rate of alpha-alanine | Cereibacter sphaeroides | 5-aminolevulinate + 5-oxo-n-pentanoate | - |
ir | |
epsilon-amino-n-caproate + 4,5-dioxopentanoate | transaminate at 103% of the rate of alpha-alanine | Cereibacter sphaeroides | 5-aminolevulinate + 6-oxo-n-hexanoate | - |
ir | |
gamma-amino-n-butyrate + 4,5-dioxopentanoate | transaminate at 122% of the rate of alpha-alanine | Cereibacter sphaeroides | 5-aminolevulinate + succinic semialdehyde | - |
ir | |
glycine + 4,5-dioxopentanoate | less than 10% of the activity of alpha-alanine | Cereibacter sphaeroides | 5-aminolevulinate + glyoxylate | - |
ir | |
L-alanine + 4,5-dioxopentanoate | - |
Cereibacter sphaeroides | 5-aminolevulinate + pyruvate | - |
ir | |
L-ornithine + 4,5-dioxopentanoate | less than 10% of the activity of alpha-alanine | Cereibacter sphaeroides | 5-aminolevulinate + 5-amino-2-oxopentanoate | - |
ir | |
additional information | ability of beta-alanine to replace alpha-alanine as amino group donor is lost during purification, alpha-amino-n-butyrate, alpha-aminoisobutyrate, beta-amino-n-butyrate and beta-aminoisobutyrate are inactive, serine, taurine, glutamine, asparagine, glutamic and aspartic acids, lysine and cysteine all have negligible activity | Cereibacter sphaeroides | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Cereibacter sphaeroides |