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Literature summary for 2.6.1.43 extracted from

  • Neuberger, A.; Turner, J.M.
    gamma, delta-Dioxovalerate aminotransferase activity in Rhodopseudomonas spheroides (1963), Biochim. Biophys. Acta, 67, 342-345.
    View publication on PubMed

General Stability

General Stability Organism
glutathione or beta-mercaptoethanol restores activity of aged enzyme preparations, optimally at 1.0 mM Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
4,5-Dioxopentanoate substrate inhibition Cereibacter sphaeroides
acetyl acetone
-
Cereibacter sphaeroides
amino-oxyacetic acid
-
Cereibacter sphaeroides
benzoyl acetone
-
Cereibacter sphaeroides
beta-Alanine 8% inhibition Cereibacter sphaeroides
beta-Iodopropionate 30% inhibition Cereibacter sphaeroides
cadaverine 14% inhibition Cereibacter sphaeroides
cysteamine
-
Cereibacter sphaeroides
cysteine 90% inhibition Cereibacter sphaeroides
diacetyl
-
Cereibacter sphaeroides
ethyl acetoacetate
-
Cereibacter sphaeroides
glyoxylic acid
-
Cereibacter sphaeroides
homocysteine
-
Cereibacter sphaeroides
iodoacetate 30% inhibition Cereibacter sphaeroides
Isonicotinic acid hydrazide
-
Cereibacter sphaeroides
lysine 20% inhibition Cereibacter sphaeroides
additional information iodoacetamide and chloroacetamide are not inhibitors Cereibacter sphaeroides
ophthalaldehyde
-
Cereibacter sphaeroides
ornithine 20% inhibition Cereibacter sphaeroides
oxaloacetic acid
-
Cereibacter sphaeroides
p-chloromercuribenzoate 78% inhibition Cereibacter sphaeroides
Penicillamine
-
Cereibacter sphaeroides
Pyruvic acid
-
Cereibacter sphaeroides
succinic acid
-
Cereibacter sphaeroides
Thioglycollate 74% inhibition Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
4,5-Dioxopentanoate pH 7.0, 37°C Cereibacter sphaeroides
8
-
L-alanine pH 7.0, 37°C Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + 4,5-dioxopentanoate Cereibacter sphaeroides
-
5-aminolevulinate + pyruvate
-
ir

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
-15°C, stable for several months Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
delta-amino-n-valerate + 4,5-dioxopentanoate transaminate at 95% of the rate of alpha-alanine Cereibacter sphaeroides 5-aminolevulinate + 5-oxo-n-pentanoate
-
ir
epsilon-amino-n-caproate + 4,5-dioxopentanoate transaminate at 103% of the rate of alpha-alanine Cereibacter sphaeroides 5-aminolevulinate + 6-oxo-n-hexanoate
-
ir
gamma-amino-n-butyrate + 4,5-dioxopentanoate transaminate at 122% of the rate of alpha-alanine Cereibacter sphaeroides 5-aminolevulinate + succinic semialdehyde
-
ir
glycine + 4,5-dioxopentanoate less than 10% of the activity of alpha-alanine Cereibacter sphaeroides 5-aminolevulinate + glyoxylate
-
ir
L-alanine + 4,5-dioxopentanoate
-
Cereibacter sphaeroides 5-aminolevulinate + pyruvate
-
ir
L-ornithine + 4,5-dioxopentanoate less than 10% of the activity of alpha-alanine Cereibacter sphaeroides 5-aminolevulinate + 5-amino-2-oxopentanoate
-
ir
additional information ability of beta-alanine to replace alpha-alanine as amino group donor is lost during purification, alpha-amino-n-butyrate, alpha-aminoisobutyrate, beta-amino-n-butyrate and beta-aminoisobutyrate are inactive, serine, taurine, glutamine, asparagine, glutamic and aspartic acids, lysine and cysteine all have negligible activity Cereibacter sphaeroides ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Cereibacter sphaeroides