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Literature summary for 2.6.1.42 extracted from
- Structural analysis of mycobacterial branched-chain aminotransferase: implications for inhibitor design (2010), Acta Crystallogr. Sect. D, 66, 549-557.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged protein expressed in Escherichia coli BL21AI | Mycobacterium tuberculosis |
| His-tagged protein expressed in Escherichia coli BL21AI | Mycolicibacterium smegmatis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop method, ammonium sulfate, PEG 3350 | Mycolicibacterium smegmatis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NH4+ | - |
Mycobacterium tuberculosis |  |
| O-Benzylhydroxylamine | - |
Mycobacterium tuberculosis | |
| O-Benzylhydroxylamine | - |
Mycolicibacterium smegmatis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycolicibacterium smegmatis | A0R066 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion affinity chromatography (Ni2+), gel filtration | Mycobacterium tuberculosis |
| immobilized metal ion affinity chromatography (Ni2+), gel filtration | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-isoleucine + 2-oxoglutarate | - |
Mycobacterium tuberculosis | 3-methyl-2-oxopentanoic acid + L-glutamate | - |
r | |
| L-isoleucine + 2-oxoglutarate | - |
Mycolicibacterium smegmatis | 3-methyl-2-oxopentanoic acid + L-glutamate | - |
r | |
| L-leucine + 2-oxoglutarate | - |
Mycobacterium tuberculosis | 4-methyl-2-oxopentanoic acid + L-glutamate | - |
r | |
| L-leucine + 2-oxoglutarate | - |
Mycolicibacterium smegmatis | 4-methyl-2-oxopentanoic acid + L-glutamate | - |
r | |
| L-valine + 2-oxoglutarate | - |
Mycobacterium tuberculosis | 3-methyl-2-oxobutanoic acid + L-glutamate | - |
r | |
| L-valine + 2-oxoglutarate | - |
Mycolicibacterium smegmatis | 3-methyl-2-oxobutanoic acid + L-glutamate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Mycobacterium tuberculosis |
| homodimer | - |
Mycolicibacterium smegmatis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| branched-chain aminotransferase | - |
Mycobacterium tuberculosis |
| branched-chain aminotransferase | - |
Mycolicibacterium smegmatis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Mycobacterium tuberculosis | |
| pyridoxal 5'-phosphate | - |
Mycolicibacterium smegmatis | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
pH 7.5, 37°C | Mycobacterium tuberculosis | NH4+ | |
| 0.042 | - |
pH 7.5, 37°C | Mycolicibacterium smegmatis | O-Benzylhydroxylamine | |
| 0.065 | - |
pH 7.5, 37°C | Mycobacterium tuberculosis | O-Benzylhydroxylamine | |
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Release 2023.1 (January 2023)
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