Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 1% (w/v) tryptone, 50 mM HEPES pH 7.0, and 12% (w/v) polyethylene glycol 3350 | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39700 | - |
2 * 39700, calculated from amino acid sequence | Mycobacterium tuberculosis |
40000 | - |
2 * 40000, the biologically active form of IlvE is a homodimer, SDS-PAGE | Mycobacterium tuberculosis |
80000 | - |
gel filtration | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WQ75 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WQ75 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ affinity column chromatography and S75 gel filtration | Mycobacterium tuberculosis |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 39700, calculated from amino acid sequence | Mycobacterium tuberculosis |
homodimer | 2 * 40000, the biologically active form of IlvE is a homodimer, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
BcaT | - |
Mycobacterium tuberculosis |
branched-chain amino-acid transaminase | - |
Mycobacterium tuberculosis |
IlvE | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Mycobacterium tuberculosis |