General Stability | Organism |
---|---|
2-mercaptoethanol protects against inactivation by sulfhydryl reagents | Homo sapiens |
2-mercaptoethanol protects against inactivation by sulfhydryl reagents | Rattus norvegicus |
2-mercaptoethanol protects against inactivation by sulfhydryl reagents | Canis lupus familiaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydrazine | - |
Canis lupus familiaris | |
hydrazine | - |
Homo sapiens | |
hydrazine | - |
Rattus norvegicus | |
hydroxylamine | - |
Canis lupus familiaris | |
hydroxylamine | - |
Homo sapiens | |
hydroxylamine | - |
Rattus norvegicus | |
KCN | - |
Homo sapiens | |
additional information | no inhibition with isonicotinic acid hydrazide or KCN | Canis lupus familiaris | |
additional information | not inhibited by isonicotinic acid and semicarbazide | Homo sapiens | |
additional information | no inhibition with isonicotinic acid hydrazide or KCN | Rattus norvegicus | |
p-chloromercuribenzoate | isoenzyme I, complete inhibition, isoenzyme III only partially inhibited | Canis lupus familiaris | |
p-chloromercuribenzoate | isoenzyme I, complete inhibition, isoenzyme III only partially inhibited | Homo sapiens | |
p-chloromercuribenzoate | 5 mM, complete inhibition | Rattus norvegicus | |
Semicarbazide | isoenzyme II is somewhat sensitive, isoenzyme I is not | Canis lupus familiaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
2-oxoglutarate | pH 8.0, 37°C, isoenzyme III, leucine as amino group donor | Homo sapiens | |
1 | - |
2-oxoglutarate | pH 8.0, 37°C, isoenzyme III, isoleucine as amino group donor | Canis lupus familiaris | |
1.1 | - |
2-oxoglutarate | pH 8.0, 37°C, isoenzyme III, isoleucine as amino group donor | Homo sapiens | |
1.4 | - |
2-oxoglutarate | pH 8.0, 37°C, isoenzyme III, leucine as amino group donor | Canis lupus familiaris | |
1.7 | - |
2-oxoglutarate | pH 8.0, 37°C, isoenzyme III, valine as amino group donor | Homo sapiens | |
1.7 | - |
2-oxoglutarate | pH 8.0, 37°C, isoenzyme III, valine as amino group donor | Canis lupus familiaris | |
2.1 | - |
2-oxoglutarate | pH 8.0, 37°C, isoenzyme I, leucine as amino group donor | Canis lupus familiaris | |
2.3 | - |
L-leucine | pH 8.0, 37°C, isoenzyme III, 2-oxoglutarate as amino group acceptor | Canis lupus familiaris | |
2.7 | - |
2-oxoglutarate | pH 8.0, 37°C, isoenzyme I, valine as amino group donor | Canis lupus familiaris | |
3 | - |
L-valine | pH 8.0, 37°C, isoenzyme III, 2-oxoglutarate as amino group acceptor | Canis lupus familiaris | |
3 | - |
L-isoleucine | pH 8.0, 37°C, isoenzyme III, 2-oxoglutarate as amino group acceptor | Homo sapiens | |
3.6 | - |
L-leucine | pH 8.0, 37°C, isoenzyme I, 2-oxoglutarate as amino group acceptor | Canis lupus familiaris | |
4 | - |
2-oxoglutarate | pH 8.0, 37°C, leucine as amino group donor | Rattus norvegicus | |
4.2 | - |
L-isoleucine | pH 8.0, 37°C, isoenzyme III, 2-oxoglutarate as amino group acceptor | Canis lupus familiaris | |
4.8 | - |
2-oxoglutarate | pH 8.0, 37°C, isoenzyme I, isoleucine as amino group donor | Homo sapiens | |
5 | - |
2-oxoglutarate | pH 8.0, 37°C, isoenzyme I, valine as amino group donor | Homo sapiens | |
6.8 | - |
L-leucine | pH 8.0, 37°C, isoenzyme III, 2-oxoglutarate as amino group acceptor | Homo sapiens | |
7.4 | - |
L-valine | pH 8.0, 37°C, isoenzyme I, 2-oxoglutarate as amino group acceptor | Canis lupus familiaris | |
7.6 | - |
2-oxoglutarate | pH 8.0, 37°C, isoenzyme I, leucine as amino group donor | Homo sapiens | |
8.3 | - |
L-valine | pH 8.0, 37°C, isoenzyme III, 2-oxoglutarate as amino group acceptor | Homo sapiens | |
10 | - |
2-oxoglutarate | pH 8.0, 37°C, isoenzyme I, isoleucine as amino group donor | Canis lupus familiaris | |
10.3 | - |
L-isoleucine | pH 8.0, 37°C, isoenzyme I, 2-oxoglutarate as amino group acceptor | Homo sapiens | |
11.1 | - |
L-leucine | pH 8.0, 37°C | Rattus norvegicus | |
12.5 | - |
2-oxoglutarate | pH 8.0, 37°C, valine as amino group donor | Rattus norvegicus | |
14.2 | - |
2-oxoglutarate | pH 8.0, 37°C, isoleucine as amino group donor | Rattus norvegicus | |
22.2 | - |
L-isoleucine | pH 8.0, 37°C | Rattus norvegicus | |
22.2 | - |
L-isoleucine | pH 8.0, 37°C, isoenzyme I, 2-oxoglutarate as amino group acceptor | Canis lupus familiaris | |
25 | - |
L-leucine | pH 8.0, 37°C, isoenzyme I, 2-oxoglutarate as amino group acceptor | Homo sapiens | |
30.8 | - |
L-valine | pH 8.0, 37°C, isoenzyme I, 2-oxoglutarate as amino group acceptor | Homo sapiens | |
143 | - |
L-valine | pH 8.0, 37°C, 2-oxoglutarate as amino group acceptor | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Rattus norvegicus |
39000 | - |
2 * 39000, isoenzyme I, SDS-PAGE | Canis lupus familiaris |
43000 | - |
2 * 43000, isoenzyme III, SDS-PAGE | Canis lupus familiaris |
68000 | - |
gel filtration | Rattus norvegicus |
79000 | - |
sucrose density gradient centrifugation | Rattus norvegicus |
80000 | - |
isoenzyme I, gel filtration | Homo sapiens |
80000 | - |
isoenzyme I, gel filtration | Canis lupus familiaris |
90000 | - |
isoenzyme III, gel filtration | Homo sapiens |
96000 | - |
isoenzyme III, gel filtration | Canis lupus familiaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | Homo sapiens | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | Rattus norvegicus | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | Rattus norvegicus | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
dog | - |
Homo sapiens | - |
human | - |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Rattus norvegicus |
- |
Canis lupus familiaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Homo sapiens | - |
pancreas | - |
Rattus norvegicus | - |
pancreas | - |
Canis lupus familiaris | - |
skeletal muscle | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.8 | - |
- |
Rattus norvegicus |
74 | - |
- |
Canis lupus familiaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | - |
Homo sapiens | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | - |
Rattus norvegicus | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | - |
Canis lupus familiaris | L-glutamate + 3-methyl-2-oxopentanoate | - |
? | |
L-leucine + 2-oxoglutarate | - |
Homo sapiens | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Rattus norvegicus | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Canis lupus familiaris | L-glutamate + 4-methyl-2-oxopentanoate | - |
? | |
L-valine + 2-oxoglutarate | - |
Homo sapiens | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Rattus norvegicus | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Rattus norvegicus | 2-oxoisopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Canis lupus familiaris | L-glutamate + 3-methyl-2-oxobutanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Homo sapiens |
dimer | 2 * 35000, SDS-PAGE | Rattus norvegicus |
dimer | 2 * 43000, isoenzyme III, SDS-PAGE | Canis lupus familiaris |
dimer | 2 * 39000, isoenzyme I, SDS-PAGE | Canis lupus familiaris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 65 | labile to heat, activity is completely lost by heating at 65°C for 1 min, about 30% is lost within 1 min at 50°C and about 70% within 10 min | Rattus norvegicus |
65 | - |
not inactivated by heating for 10 min | Homo sapiens |
65 | - |
not inactivated by heating for 10 min | Canis lupus familiaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Rattus norvegicus |
8 | - |
isoenzyme III | Homo sapiens |
8.5 | 9.5 | isoenzyme III | Canis lupus familiaris |
9 | - |
isoenzyme I | Homo sapiens |
9 | - |
isoenzyme I | Canis lupus familiaris |