General Stability | Organism |
---|---|
pyridoxal 5'-phosphate and mercaptoethanol stabilizes the heart enzyme, addition of 2-oxoglutarate does not increase its stability | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
isoleucine | competitive inhibition of enzyme activity with leucine | Sus scrofa | |
p-chloromercuribenzoate | - |
Rattus norvegicus | |
p-chloromercuribenzoate | 0.1 mM, complete inhibition | Sus scrofa | |
valine | competitive inhibition of enzyme activity with leucine | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.63 | - |
2-oxoglutarate | pH 8.6, 25°C, heart enzyme | Sus scrofa | |
3.8 | - |
L-leucine | pH 8.6, 25°C | Sus scrofa | |
3.8 | - |
L-isoleucine | pH 8.6, 25°C | Sus scrofa | |
11 | - |
L-valine | pH 8.6, 25°C | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
cytoplasm | - |
Sus scrofa | 5737 | - |
microsome | - |
Rattus norvegicus | - |
- |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
mitochondrion | - |
Sus scrofa | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | Rattus norvegicus | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | Sus scrofa | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | Rattus norvegicus | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | Sus scrofa | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | Rattus norvegicus | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | Sus scrofa | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
pig, hog | - |
Purification (Comment) | Organism |
---|---|
hog heart, partial | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | heart muscle | Rattus norvegicus | - |
heart | heart muscle | Sus scrofa | - |
kidney | - |
Rattus norvegicus | - |
kidney | - |
Sus scrofa | - |
liver | only slight activity | Rattus norvegicus | - |
liver | only slight activity | Sus scrofa | - |
skeletal muscle | - |
Rattus norvegicus | - |
skeletal muscle | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.82 | - |
heart muscle enzyme, substrate L-leucine | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | - |
Rattus norvegicus | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | - |
Sus scrofa | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Rattus norvegicus | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Sus scrofa | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-norleucine + 2-oxoglutarate | - |
Rattus norvegicus | 2-oxohexanoate + L-glutamate | - |
r | |
L-norleucine + 2-oxoglutarate | - |
Sus scrofa | 2-oxohexanoate + L-glutamate | - |
r | |
L-norvaline + 2-oxoglutarate | heart enzyme | Sus scrofa | 2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Rattus norvegicus | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Sus scrofa | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
additional information | L-isoleucine is the best substrate for the heart enzyme, L-alanine, aspartic, alpha-amino-butyric acid and gamma-aminobutyric acid, epsilon-aminocaproic acid, L-ornithine, L-methionine and L-phenylalanine are no substrates, pyruvate is not a good acceptor | Sus scrofa | ? | - |
? | |
additional information | L-leucine is the best substrate for the heart enzyme | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
branched chain amino acid: 2-oxoglutarate aminotransferase EC 2.6.1 | formerly | Rattus norvegicus |
branched chain amino acid: 2-oxoglutarate aminotransferase EC 2.6.1 | formerly | Sus scrofa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | - |
Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | enzyme is inactivated by heat | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
- |
Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
hog heart enzyme is rather unstable at pH 8.0 | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Rattus norvegicus | |
pyridoxal 5'-phosphate | Km 0.067 mM | Sus scrofa |