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Literature summary for 2.6.1.41 extracted from

  • Mapson, L.W.; March, J.F.; Wardale, D.A.
    Biosynthesis of ethylene. 4-methylmercapto-2-oxobutyric acid: an intermediate in the formation from methionine (1969), Biochem. J., 115, 653-661.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Brassica sp. 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-methionine + pyruvate Brassica sp. involved in ethylene synthesis from methionine 4-methylthio-2-oxobutanoate + L-alanine
-
?

Organism

Organism UniProt Comment Textmining
Brassica sp.
-
cauliflower
-

Purification (Commentary)

Purification (Comment) Organism
partial Brassica sp.

Source Tissue

Source Tissue Comment Organism Textmining
floret
-
Brassica sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-methionine + 2-oxoglutarate less effective than pyruvate Brassica sp. 4-methylthio-2-oxobutanoate + L-glutamate
-
?
D-methionine + oxaloacetate less effective than pyruvate Brassica sp. 4-methylthio-2-oxobutanoate + L-aspartate
-
?
D-methionine + pyruvate
-
Brassica sp. 4-methylthio-2-oxobutanoate + L-alanine
-
?
D-methionine + pyruvate involved in ethylene synthesis from methionine Brassica sp. 4-methylthio-2-oxobutanoate + L-alanine
-
?
L-methionine + pyruvate one third of activity compared to D-methionine Brassica sp. 4-methylthio-2-oxobutanoate + L-alanine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Brassica sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Brassica sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 8 15% of maximal activity at pH 6.0, 66% of maximal activity at pH 8.0 Brassica sp.