General Stability | Organism |
---|---|
0.1 mM dithiothreitol improves the stability of the enzyme by 50% | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-methylglutaric acid | 6 mM, 2% inhibition | Bos taurus | |
3-methylglutaric acid | 6 mM, 38% inhibition | Rattus norvegicus | |
adipic acid | 6 mM, 48% inhibition | Bos taurus | |
adipic acid | 6 mM, 60% inhibition | Rattus norvegicus | |
azelaic acid | 6 mM, 36% inhibition | Bos taurus | |
azelaic acid | 6 mM, 38% inhibition | Rattus norvegicus | |
Decanoic acid | 6 mM, 50% inhibition | Bos taurus | |
Decanoic acid | 6 mM, 45% inhibition | Rattus norvegicus | |
Diethylglutaric acid | 6 mM, 20% inhibition | Bos taurus | |
Diethylglutaric acid | 6 mM, 20% inhibition | Rattus norvegicus | |
dimethylglutaric acid | 6 mM, 15% inhibition | Bos taurus | |
dimethylglutaric acid | 6 mM, 42% inhibition | Rattus norvegicus | |
Glutaric acid | 6 mM, 5% inhibition | Rattus norvegicus | |
Kynurenic acid | 1 mM, 30% inhibition | Bos taurus | |
Kynurenic acid | 1 mM, 40% inhibition | Rattus norvegicus | |
additional information | oxaloacetic acid is no inhibitor | Bos taurus | |
additional information | oxaloacetic acid is no inhibitor | Rattus norvegicus | |
pimelic acid | 6 mM, 28% inhibition | Bos taurus | |
pimelic acid | 6 mM, 32% inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
2-oxoadipic acid | pH 7.0, 37°C | Rattus norvegicus | |
0.5 | - |
2-oxoadipic acid | pH 7.0, 37°C | Bos taurus | |
4.6 | - |
L-Glutamic acid | pH 7.0, 37°C | Rattus norvegicus | |
4.6 | - |
L-Glutamic acid | pH 7.0, 37°C | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
cytoplasm | - |
Bos taurus | 5737 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
2 * 41000, SDS-PAGE | Rattus norvegicus |
41000 | - |
2 * 41000, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-aminoadipate + 2-oxoglutarate | Rattus norvegicus | lysine catabolic pathway | 2-oxoadipate + L-glutamate | - |
r | |
L-2-aminoadipate + 2-oxoglutarate | Bos taurus | lysine catabolic pathway | 2-oxoadipate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
bovine | - |
Rattus norvegicus | - |
Sprague-Dawley | - |
Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
kidney | - |
Bos taurus | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.53 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
4°C, partially purified enzyme in 8 mM potassium phosphate buffer, pH 7.1, 100% activity is lost when stored for 14 days | Bos taurus |
4°C, partially purified enzyme in 8 mM potassium phosphate buffer, pH 7.1, 15% activity is lost when stored for 14 days | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminoadipate + pyruvate | - |
Rattus norvegicus | 2-oxoadipate + L-alanine | - |
r | |
2-aminoadipate + pyruvate | - |
Bos taurus | 2-oxoadipate + L-alanine | - |
r | |
3,5-di-iodotyrosine + 2-oxoglutarate | - |
Rattus norvegicus | 3,5-diiodophenylpyruvate + L-glutamate | - |
r | |
3,5-di-iodotyrosine + 2-oxoglutarate | - |
Bos taurus | 3,5-diiodophenylpyruvate + L-glutamate | - |
r | |
DL-2-aminopimelic acid + 2-oxoglutarate | - |
Rattus norvegicus | 2-oxoadipate + L-glutamate | - |
r | |
DL-2-aminopimelic acid + 2-oxoglutarate | - |
Bos taurus | 2-oxoadipate + L-glutamate | - |
r | |
kynurenine + 2-oxoglutarate | - |
Rattus norvegicus | kynurenic acid + L-glutamate | - |
ir | |
L-2-aminoadipate + 2-oxoglutarate | - |
Rattus norvegicus | 2-oxoadipate + L-glutamate | - |
r | |
L-2-aminoadipate + 2-oxoglutarate | - |
Bos taurus | 2-oxoadipate + L-glutamate | - |
r | |
L-2-aminoadipate + 2-oxoglutarate | lysine catabolic pathway | Rattus norvegicus | 2-oxoadipate + L-glutamate | - |
r | |
L-2-aminoadipate + 2-oxoglutarate | lysine catabolic pathway | Bos taurus | 2-oxoadipate + L-glutamate | - |
r | |
L-histidine + 2-oxoglutarate | - |
Rattus norvegicus | 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate | - |
r | |
L-histidine + 2-oxoglutarate | - |
Bos taurus | 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate | - |
r | |
L-histidine + 2-oxoglutarate | - |
Rattus norvegicus Sprague-Dawley | 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate | - |
r | |
L-histidine + pyruvate | - |
Rattus norvegicus | 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine | - |
r | |
L-histidine + pyruvate | - |
Bos taurus | 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine | - |
r | |
L-histidine + pyruvate | - |
Rattus norvegicus Sprague-Dawley | 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine | - |
r | |
L-norleucine + 2-oxoglutarate | - |
Rattus norvegicus | 2-oxohexanoate + L-glutamate | - |
r | |
L-norleucine + 2-oxoglutarate | - |
Bos taurus | 2-oxohexanoate + L-glutamate | - |
r | |
L-norleucine + 2-oxoglutarate | - |
Rattus norvegicus Sprague-Dawley | 2-oxohexanoate + L-glutamate | - |
r | |
additional information | only slight activity with phenylalanine, tryptophan, tyrosine, aspartate or alanine as amino group donors, glyoxylate is no amino group acceptor | Rattus norvegicus | ? | - |
? | |
additional information | only slight activity with phenylalanine, tryptophan, tyrosine, aspartate or alanine as amino group donors, glyoxylate is no amino group acceptor | Bos taurus | ? | - |
? | |
additional information | no kynurenine aminotransferase activity | Bos taurus | ? | - |
? | |
additional information | 2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein | Rattus norvegicus | ? | - |
? | |
additional information | only slight activity with phenylalanine, tryptophan, tyrosine, aspartate or alanine as amino group donors, glyoxylate is no amino group acceptor | Rattus norvegicus Sprague-Dawley | ? | - |
? | |
additional information | 2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein | Rattus norvegicus Sprague-Dawley | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41000, SDS-PAGE | Rattus norvegicus |
dimer | 2 * 41000, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
AadAT | - |
Rattus norvegicus |
AadAT | - |
Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rattus norvegicus |
7 | - |
- |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Rattus norvegicus | |
pyridoxal 5'-phosphate | - |
Bos taurus |