Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-Methyl-DL-cysteine | inhibits transamination of cysteine | Rattus norvegicus | |
Maleate | - |
Rattus norvegicus | |
sodium phosphate buffer | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83500 | - |
gel filtration | Rattus norvegicus |
84000 | - |
1 * 84000, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + 2-oxoglutarate | Rattus norvegicus | important part of enzyme together with beta-mercaptopyruvate sulfurtransferase in the regulation of thiosulfate formation | L-glutamate + beta-mercaptopyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
TEAE-cellulose, isoelectric focusing | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.085 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20ºC, 68% inactivated after 32 days | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + 2-oxoglutarate | - |
Rattus norvegicus | L-glutamate + beta-mercaptopyruvate | - |
? | |
L-cysteine + 2-oxoglutarate | important part of enzyme together with beta-mercaptopyruvate sulfurtransferase in the regulation of thiosulfate formation | Rattus norvegicus | L-glutamate + beta-mercaptopyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 84000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
1 h at room temperature, complete inactivation | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | about 60% of activity maximum at pH 7 and 11 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | pyridoxal phosphate protein | Rattus norvegicus |