Literature summary for 2.6.1.2 extracted from

Donini, S.; Ferrari, M.; Fedeli, C.; Faini, M.; Lamberto, I.; Marletta, A.S.; Mellini, L.; Panini, M.; Percudani, R.; Pollegioni, L.; Caldinelli, L.; Petrucco, S.; Peracchi, A.
Recombinant production of eight human cytosolic aminotransferases and assessment of their potential involvement in glyoxylate metabolism (2009), Biochem. J., 422, 265-272.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed as His6-tagged protein in Escherichia coli cells	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.75	-	glyoxylate	pH 7.4, 37°C, cosubstrate: L-glutamate	Homo sapiens
0.81	-	glyoxylate	pH 7.4, 37°C, cosubstrate: L-alanine	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
57000	-	recombinant enzyme	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P24298	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.1	-	pH 7.4, 25°C, L-alanine (10 mM) + glyoxylate	Homo sapiens
2.5	-	pH 7.4, 25°C, L-glutamate (10 mM) + glyoxylate	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-alanine + glyoxylate	L-glutamate and L-alanine are by far the best amino-group donors	Homo sapiens	pyruvate + glycine	-	?
L-glutamate + glyoxylate	L-glutamate and L-alanine are by far the best amino-group donors	Homo sapiens	2-oxoglutarate + glycine	-	?

Synonyms

Synonyms	Comment	Organism
GPT	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Homo sapiens
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Homo sapiens

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Release 2023.1 (January 2023)