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Literature summary for 2.6.1.2 extracted from

  • Liu, L.; Zhong, S.; Yang, R.; Hu, H.; Yu, D.; Zhu, D.; Hua, Z.; Shuldiner, A.R.; Goldstein, R.; Reagan, W.J.; Gong, D.W.
    Expression, purification, and initial characterization of human alanine aminotransferase (ALT) isoenzyme 1 and 2 in High-five insect cells (2008), Protein Expr. Purif., 60, 225-231.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 cell Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
x * 59000, calculated, x * 60000, SDS-PAGE, His-tagged protein Homo sapiens
60000
-
x * 59000, calculated, x * 60000, SDS-PAGE, His-tagged protein Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
isoform Alt1
-
Homo sapiens
-
isoform Alt2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4
-
25°C Homo sapiens
60.2
-
25°C Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 20 mM Tris-HCl, pH 7.5, freezing is worse than storage at 4°C or 25°C Homo sapiens
-80°C, 20 mM Tris-HCl, pH 7.5, 20% loss of activity in first two days, thereafter stable for at least 20 days. Addition of 25% glycerol preserves most of the activity Homo sapiens
-80°C, 20 mM Tris-HCl, pH 7.5, stable for at least 20 days. Addition of 25% glycerol preserves most of the activity Homo sapiens
25°C, 20 mM Tris-HCl, pH 7.5, 56% loss of activity in first two days. Addition of 25% glycerol preserves most of the activity Homo sapiens
25°C, 20 mM Tris-HCl, pH 7.5, 80-90% residual activity after 10 days, 60% residual activity after 20 days. Addition of 25% glycerol preserves most of the activity Homo sapiens
37°C, 20 mM Tris-HCl, pH 7.5, 50% loss of activity in 24 h, and complete loss of activity within one week Homo sapiens
37°C, 20 mM Tris-HCl, pH 7.5, 72% loss of activity in first two days Homo sapiens
4°C, 20 mM Tris-HCl, pH 7.5, 53% loss of activity in first two days. Addition of 25% glycerol preserves most of the activity Homo sapiens
4°C, 20 mM Tris-HCl, pH 7.5, stable for at least 20 days. Addition of 25% glycerol preserves most of the activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + 2-oxoglutarate
-
Homo sapiens pyruvate + L-glutamate
-
?

Subunits

Subunits Comment Organism
? x * 59000, calculated, x * 60000, SDS-PAGE, His-tagged protein Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 almost full activity within this range Homo sapiens