Cloned (Comment) | Organism |
---|---|
expression in Sf9 cell | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
x * 59000, calculated, x * 60000, SDS-PAGE, His-tagged protein | Homo sapiens |
60000 | - |
x * 59000, calculated, x * 60000, SDS-PAGE, His-tagged protein | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isoform Alt1 | - |
Homo sapiens | - |
isoform Alt2 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
25°C | Homo sapiens |
60.2 | - |
25°C | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, 20 mM Tris-HCl, pH 7.5, freezing is worse than storage at 4°C or 25°C | Homo sapiens |
-80°C, 20 mM Tris-HCl, pH 7.5, 20% loss of activity in first two days, thereafter stable for at least 20 days. Addition of 25% glycerol preserves most of the activity | Homo sapiens |
-80°C, 20 mM Tris-HCl, pH 7.5, stable for at least 20 days. Addition of 25% glycerol preserves most of the activity | Homo sapiens |
25°C, 20 mM Tris-HCl, pH 7.5, 56% loss of activity in first two days. Addition of 25% glycerol preserves most of the activity | Homo sapiens |
25°C, 20 mM Tris-HCl, pH 7.5, 80-90% residual activity after 10 days, 60% residual activity after 20 days. Addition of 25% glycerol preserves most of the activity | Homo sapiens |
37°C, 20 mM Tris-HCl, pH 7.5, 50% loss of activity in 24 h, and complete loss of activity within one week | Homo sapiens |
37°C, 20 mM Tris-HCl, pH 7.5, 72% loss of activity in first two days | Homo sapiens |
4°C, 20 mM Tris-HCl, pH 7.5, 53% loss of activity in first two days. Addition of 25% glycerol preserves most of the activity | Homo sapiens |
4°C, 20 mM Tris-HCl, pH 7.5, stable for at least 20 days. Addition of 25% glycerol preserves most of the activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + 2-oxoglutarate | - |
Homo sapiens | pyruvate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59000, calculated, x * 60000, SDS-PAGE, His-tagged protein | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | almost full activity within this range | Homo sapiens |