Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aminooxyacetic acid | 2.5 mM, complete inhibition | Rattus norvegicus | |
Glutarate | 2.5 mM, 69% inhibition | Rattus norvegicus | |
HgCl2 | 2.5 mM, 96% inhibition | Rattus norvegicus | |
hydroxylamine | 2.5 mM, complete inhibition | Rattus norvegicus | |
Maleate | 2.5 mM, 70% inhibition | Rattus norvegicus | |
additional information | not inhibited by EDTA, o-phenanthroline and L-ascorbate | Rattus norvegicus | |
p-hydroxymercuribenzoate | 2.5 mM, complete inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
2-oxoglutarate | 200 mM L-alanine and 2 mM 2-oxoglutarate in 0.1 M phosphate buffer, pH 7.4, at 37°C | Rattus norvegicus | |
0.51 | - |
L-alanine | 200 mM L-alanine and 2 mM 2-oxoglutarate in 0.1 M phosphate buffer, pH 7.4, at 37°C | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17700 | - |
SDS-PAGE | Rattus norvegicus |
18300 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sephadex G-150 column chromatography, DEAE-Sephadex A-25 column chromatography, and hydroxyapatite column chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-alanine + 2-oxoglutarate | 63% specific activity compared to L-alanine | Rattus norvegicus | pyruvate + L-glutamate | - |
? | |
L-alanine + 2-oxoglutarate | - |
Rattus norvegicus | pyruvate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | 6% specific activity compared to L-alanine | Rattus norvegicus | 2-oxobutan-1,4-dioate + L-glutamate | - |
? | |
L-leucine + 2-oxoglutarate | 9% specific activity compared to L-alanine | Rattus norvegicus | 2-oxo-4-methylpentanoate + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | 12.5% specific activity compared to L-alanine | Rattus norvegicus | (4-hydroxyphenyl)pyruvate + L-glutamate | - |
? | |
additional information | no specificity for L-glutamate, L-cysteine, L-glycine, L-methionine, L-isoleucine, L-threonine, L-valine, L-histidine, beta-alanine, L-arginine hydrochloride, L-cysteine hydrochloride, L-hydroxyproline, DL-phenylalanine, DL-threonine, DL-aspartate, DL-isoleucine, DL-tryptophan, DL-norleucine, DL-histidine hydrochloride, DL-homocysteine, L-beta-phenylalanine | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AlaAT | - |
Rattus norvegicus |
alanine-2-oxoglutarate aminotransferase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.1 | - |
L-alanine | 200 mM L-alanine and 2 mM 2-oxoglutarate in 0.1 M phosphate buffer, pH 7.4, at 37°C | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | activates the enzyme with a Km of 0.057 mM | Rattus norvegicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | isoelectrofocusing | - |
4.2 |