Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
2-oxoglutarate | pH 7.2, 80°C, native enzyme | Pyrococcus furiosus | |
1.1 | - |
2-oxoglutarate | pH 7.2, 80°C, recombinant enzyme | Pyrococcus furiosus | |
2.8 | - |
alanine | pH 7.2, 80°C, native enzyme | Pyrococcus furiosus | |
3.2 | - |
L-alanine | pH 7.2, 80°C, recombinant enzyme | Pyrococcus furiosus | |
4.3 | - |
glutamate | pH 7.2, 80°C, native enzyme | Pyrococcus furiosus | |
4.7 | - |
glutamate | pH 7.2, 80°C, recombinant enzyme | Pyrococcus furiosus | |
5.4 | - |
pyruvate | pH 7.2, 80°C, native enzyme | Pyrococcus furiosus | |
5.6 | - |
pyruvate | pH 7.2, 80°C, recombinant enzyme | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
2 * 46000, SDS-PAGE | Pyrococcus furiosus |
93400 | - |
gel filtration | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q9P9M8 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme, Q-Sepharose, phenyl-Sepharose, hydroxyapatite, S-Sepharose, Mono Q, Superdex 200, recombinant enzyme, heat treatment, Mono Q | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
158 | - |
native enzyme | Pyrococcus furiosus |
243 | - |
recombinant enzyme | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aspartate + 2-oxoglutarate | 14% of activity with glutamate and pyruvate | Pyrococcus furiosus | malate + L-glutamate | - |
? | |
aspartate + pyruvate | 10% of activity with alanine and 2-oxoglutarate | Pyrococcus furiosus | malate + alanine | - |
? | |
L-alanine + 2-oxoglutarate | - |
Pyrococcus furiosus | pyruvate + L-glutamate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 46000, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
above 95°C, native and recombinant enzyme | Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 95 | native and recombinant enzyme | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.8 | native and recombinant enzyme | Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.2 | native and recombinant enzyme | Pyrococcus furiosus |