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Literature summary for 2.6.1.2 extracted from

  • De Rosa, G.; Burk, T.L.; Swick, R.W.
    Isolation and characterization of mitochondrial alanine aminotransferase from porcine tissue (1979), Biochim. Biophys. Acta, 567, 116-124.
    View publication on PubMed
Search for more literature for 2.6.1.2

General Stability

General Stability Organism
enzyme does not tolerate freezing at any stage after sonication of the isolated mitochondria Sus scrofa
K+ or L-alanine stabilizes Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Aminooxyacetate 0.0004 mM, 85% inhibition of mitochondrial alanine aminotransferase Sus scrofa
cycloserine 1 mM, 10 mM, 50 mM, 10%, 52% and 100% inhibition respectively Sus scrofa
D-alanine competitive vs. L-alanine Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 pyruvate pH 7.8, 30°C, mitochondrial alanine aminotransferase Sus scrofa
0.4
-
 2-oxoglutarate pH 7.8, 30°C, mitochondrial alanine aminotransferase Sus scrofa
2
-
 alanine pH 7.8, 30°C, mitochondrial alanine aminotransferase Sus scrofa
32
-
 glutamate pH 7.8, 30°C, mitochondrial alanine aminotransferase Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Sus scrofa 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 1 * 75000, SDS-PAGE Sus scrofa
80000
-
 gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sephadex G-150, DEAE-cellulose, ammonium sulfate, isoelectric focusing Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
L-alanine + 2-oxoglutarate = pyruvate + L-glutamate ping pong type reaction Sus scrofa
a

Source Tissue

Source Tissue Comment Organism Textmining
kidney cortex Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.55
-
-
 Sus scrofa
213
-
-
 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + 2-oxoglutarate D-alanine at 2% of the activity with L-alanine Sus scrofa pyruvate + L-glutamate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 75000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
3.5 9.5 active over whole range Sus scrofa