General Stability | Organism |
---|---|
during the purification procedure the enzyme needs protective substances, i.e. glycerol, 2-mercaptoethanol and alanine | Solanum lycopersicum |
uneffected by repeated freezing and thawing | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
pyruvate | pH 7.25, 37°C | Solanum lycopersicum | |
0.28 | - |
2-oxoglutarate | pH 7.25, 37°C | Solanum lycopersicum | |
2.3 | - |
glutamate | pH 7.25, 37°C | Solanum lycopersicum | |
2.8 | - |
alanine | pH 7.25, 37°C | Solanum lycopersicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Solanum lycopersicum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
gel filtration | Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
tomato, 2 alanine aminotransferases, soluble and Triton X-100 extractable | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-Sephadex, Sephadex G-200, partial purification | Solanum lycopersicum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-alanine + 2-oxoglutarate = pyruvate + L-glutamate | ping pong bi bi mechanism | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Solanum lycopersicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
120 | - |
- |
Solanum lycopersicum |
Storage Stability | Organism |
---|---|
-20°C, stable for several months | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + 2-oxoglutarate | rate of the forward reaction is 4 times higher than the rate of the reverse reaction, no activity with aspartate, glycine, threonine, leucine, methionine, arginine, phenylalanine, tyrosine, tryptophan, glyoxylate and 2-oxo-3-methylvalerate | Solanum lycopersicum | pyruvate + L-glutamate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Solanum lycopersicum |
37 | - |
assay at | Solanum lycopersicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
quickly inactivated, 100 mM alanine gives slight protection | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | 7.5 | - |
Solanum lycopersicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | rapid decrease of activity below pH 7 and above pH 7.5 | Solanum lycopersicum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
strong loss of activity below | Solanum lycopersicum |