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Literature summary for 2.6.1.19 extracted from

  • Clark, S.M.; Di Leo, R.; Dhanoa, P.K.; Van Cauwenberghe, O.R.; Mullen, R.T.; Shelp, B.J.
    Biochemical characterization, mitochondrial localization, expression, and potential functions for an Arabidopsis gamma-aminobutyrate transaminase that utilizes both pyruvate and glyoxylate (2009), J. Exp. Bot., 60, 1743-1757.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.19

Cloned(Commentary)

Cloned (Comment) Organism
co-expression of the truncated GABA-T cDNA with the GroES/ELmolecular chaperone complex in Escherichia coli BL-21(DE3) Rosetta (pLysS) cells, and GABA-T is expressed in Nicotiana tabacum BY-2 cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
beta-Alanine competitive inhibitor of pyruvate-dependent GABA-T activity Arabidopsis thaliana
glycine competitive inhibitor of pyruvate-dependent GABA-T activity Arabidopsis thaliana
ornithine competitive inhibitor of pyruvate-dependent GABA-T activity Arabidopsis thaliana
vigabatrin competitive inhibitor of pyruvate-dependent GABA-T activity Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 4-Oxobutanoate with L-alanine as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana
0.11
-
 glyoxylate in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana
0.14
-
 pyruvate in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana
0.18
-
 4-aminobutanoate with glyoxylate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana
0.34
-
 4-aminobutanoate with pyruvate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana
2.4
-
 L-alanine with 4-oxobutanoate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Arabidopsis thaliana 5739
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q94CE5 subunit
-

Purification (Commentary)

Purification (Comment) Organism
ProBond nickel resin column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutanoate + glyoxylate
-
 Arabidopsis thaliana 4-oxobutanoate + glycine
-
 ?
4-aminobutanoate + glyoxylate
-
 Arabidopsis thaliana Wassilewskija 4-oxobutanoate + glycine
-
 ?
4-aminobutanoate + glyoxylate
-
 Arabidopsis thaliana Col-0 4-oxobutanoate + glycine
-
 ?
4-aminobutanoate + pyruvate
-
 Arabidopsis thaliana 4-oxobutanoate + L-alanine
-
 ?
4-aminobutanoate + pyruvate
-
 Arabidopsis thaliana Wassilewskija 4-oxobutanoate + L-alanine
-
 ?
4-aminobutanoate + pyruvate
-
 Arabidopsis thaliana Col-0 4-oxobutanoate + L-alanine
-
 ?
additional information cannot utilize 2-oxoglutarate as amino acceptor Arabidopsis thaliana ?
-
 ?
additional information cannot utilize 2-oxoglutarate as amino acceptor Arabidopsis thaliana Wassilewskija ?
-
 ?
additional information cannot utilize 2-oxoglutarate as amino acceptor Arabidopsis thaliana Col-0 ?
-
 ?

Synonyms

Synonyms Comment Organism
GABA-T
-
 Arabidopsis thaliana
gamma-aminobutyrate transaminase
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.9
-
 4-aminobutanoate with glyoxylate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana
9.7
-
 glyoxylate in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana
10.6
-
 pyruvate in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana
10.6
-
 4-aminobutanoate with pyruvate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana
10.8
-
 4-Oxobutanoate with L-alanine as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana
15.4
-
 L-alanine with 4-oxobutanoate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 for pyruvate-dependent GABA-T activity Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
8.5 10
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.46
-
 ornithine
-
 Arabidopsis thaliana
0.55
-
 beta-Alanine
-
 Arabidopsis thaliana
0.62
-
 vigabatrin
-
 Arabidopsis thaliana
3.7
-
 glycine
-
 Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana expression is highest in roots and increases as a function of leaf development up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.4
-
 L-alanine with 4-oxobutanoate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana
31
-
 4-aminobutanoate with pyruvate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana
39
-
 4-aminobutanoate with glyoxylate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana
76
-
 pyruvate in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana
88
-
 glyoxylate in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana
770
-
 4-Oxobutanoate with L-alanine as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C Arabidopsis thaliana