Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | - |
Pseudomonas fluorescens | |
KCN | - |
Pseudomonas fluorescens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Pseudomonas fluorescens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanoate + 2-oxoglutarate | Pseudomonas fluorescens | inducible enzyme | 4-oxobutanoate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.4 | - |
- |
Pseudomonas fluorescens |
Storage Stability | Organism |
---|---|
-20°C, several months | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanoate + 2-oxoglutarate | - |
Pseudomonas fluorescens | 4-oxobutanoate + L-glutamate | - |
r | |
4-aminobutanoate + 2-oxoglutarate | inducible enzyme | Pseudomonas fluorescens | 4-oxobutanoate + L-glutamate | - |
? | |
additional information | overview | Pseudomonas fluorescens | ? | - |
? | |
additional information | no amino acceptors are oxaloacetate, pyruvate | Pseudomonas fluorescens | ? | - |
? | |
additional information | no substrate: lysine | Pseudomonas fluorescens | ? | - |
? | |
additional information | no amino acceptors are ketomalonic acid, alpha-ketoisovaleric acid | Pseudomonas fluorescens | ? | - |
? | |
additional information | aspartate, L-2,4-diaminoglutarate, alanine, D-glutamate or malonic semialdehyde cannot replace L-glutamate or succinic semialdehyde in the reverse reaction | Pseudomonas fluorescens | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas fluorescens |
8.6 | 9 | at 25°C | Pseudomonas fluorescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9.2 | about half-maximal activity at pH 8 | Pseudomonas fluorescens |
8 | 9.2 | about 60% of maximal activity at pH 9.2 | Pseudomonas fluorescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | below and above, rapid inactivation | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | no activation by addition of pyridoxal phosphate, but sensitive to carbonyl reagents | Pseudomonas fluorescens |