Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21gold(DE3) cells | Pseudomonas aeruginosa |
General Stability | Organism |
---|---|
the rate of the reaction with the purified enzyme in 100 mM potassium phosphate buffer, pH 7.0 with 2 mM pyridoxal phosphate cofactor at 25°C slows markedly after 50 h with little further product formation up to 75 h | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
4 * 43000 | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-alanine + pyruvate | Pseudomonas aeruginosa | - |
malonic semialdehyde + L-alanine | - |
? | |
beta-alanine + pyruvate | Pseudomonas aeruginosa PAO2 | - |
malonic semialdehyde + L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel column chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-alpha-methylbenzylamine + pyruvate | - |
Pseudomonas aeruginosa | acetophenone + L-alanine | - |
? | |
(S)-alpha-methylbenzylamine + pyruvate | - |
Pseudomonas aeruginosa PAO2 | acetophenone + L-alanine | - |
? | |
beta-alanine + pyruvate | - |
Pseudomonas aeruginosa | malonic semialdehyde + L-alanine | - |
? | |
beta-alanine + pyruvate | - |
Pseudomonas aeruginosa PAO2 | malonic semialdehyde + L-alanine | - |
? | |
L-erythrulose + pyruvate | - |
Pseudomonas aeruginosa | 2-amino-1,3,4-butanetriol + ? | - |
? | |
L-erythrulose + pyruvate | - |
Pseudomonas aeruginosa PAO2 | 2-amino-1,3,4-butanetriol + ? | - |
? | |
additional information | D-methylbenzylamine is not accepted as an amine donor | Pseudomonas aeruginosa | ? | - |
? | |
additional information | D-methylbenzylamine is not accepted as an amine donor | Pseudomonas aeruginosa PAO2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 43000 | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
beta-A:P TAm | - |
Pseudomonas aeruginosa |
beta-alanine:pyruvate transaminase | - |
Pseudomonas aeruginosa |
PAO2 | - |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
the rate of the reaction with the purified enzyme in 100 mM potassium phosphate buffer, pH 7.0 with 2 mM pyridoxal phosphate cofactor at 25°C slows markedly after 50 h with little further product formation up to 75 h | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
for reactions with (S)-alpha-methylbenzylamine and L-erythrulose | Pseudomonas aeruginosa |
8.5 | - |
above pH 8.5, for reactions with the natural substrates beta-alanine and pyruvate | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas aeruginosa |