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Literature summary for 2.6.1.18 extracted from

  • Ingram, C.U.; Bommer, M.; Smith, M.E.; Dalby, P.A.; Ward, J.M.; Hailes, H.C.; Lye, G.J.
    One-pot synthesis of amino-alcohols using a de-novo transketolase and beta-alanine:pyruvate transaminase pathway in Escherichia coli (2006), Biotechnol. Bioeng., 96, 559-569.
    View publication on PubMed
Search for more literature for 2.6.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21gold(DE3) cells Pseudomonas aeruginosa

General Stability

General Stability Organism
the rate of the reaction with the purified enzyme in 100 mM potassium phosphate buffer, pH 7.0 with 2 mM pyridoxal phosphate cofactor at 25°C slows markedly after 50 h with little further product formation up to 75 h Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 4 * 43000 Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-alanine + pyruvate Pseudomonas aeruginosa
-
 malonic semialdehyde + L-alanine
-
 ?
beta-alanine + pyruvate Pseudomonas aeruginosa PAO2
-
 malonic semialdehyde + L-alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-alpha-methylbenzylamine + pyruvate
-
 Pseudomonas aeruginosa acetophenone + L-alanine
-
 ?
(S)-alpha-methylbenzylamine + pyruvate
-
 Pseudomonas aeruginosa PAO2 acetophenone + L-alanine
-
 ?
beta-alanine + pyruvate
-
 Pseudomonas aeruginosa malonic semialdehyde + L-alanine
-
 ?
beta-alanine + pyruvate
-
 Pseudomonas aeruginosa PAO2 malonic semialdehyde + L-alanine
-
 ?
L-erythrulose + pyruvate
-
 Pseudomonas aeruginosa 2-amino-1,3,4-butanetriol + ?
-
 ?
L-erythrulose + pyruvate
-
 Pseudomonas aeruginosa PAO2 2-amino-1,3,4-butanetriol + ?
-
 ?
additional information D-methylbenzylamine is not accepted as an amine donor Pseudomonas aeruginosa ?
-
 ?
additional information D-methylbenzylamine is not accepted as an amine donor Pseudomonas aeruginosa PAO2 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 43000 Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
beta-A:P TAm
-
 Pseudomonas aeruginosa
beta-alanine:pyruvate transaminase
-
 Pseudomonas aeruginosa
PAO2
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 the rate of the reaction with the purified enzyme in 100 mM potassium phosphate buffer, pH 7.0 with 2 mM pyridoxal phosphate cofactor at 25°C slows markedly after 50 h with little further product formation up to 75 h Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 for reactions with (S)-alpha-methylbenzylamine and L-erythrulose Pseudomonas aeruginosa
8.5
-
 above pH 8.5, for reactions with the natural substrates beta-alanine and pyruvate Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Pseudomonas aeruginosa